Selection of the HPLC Method in Chemical Analysis

Selection of the HPLC Method in Chemical Analysis serves as a practical guide to users of high-performance liquid chromatography and provides criteria for method selection, development, and validation.

High-performance liquid chromatography (HPLC) is the most common analytical technique currently practiced in chemistry. However, the process of finding the appropriate information for a particular analytical project requires significant effort and pre-existent knowledge in the field. Further, sorting through the wealth of published data and literature takes both time and effort away from the critical aspects of HPLC method selection.

For the first time, a systematic approach for sorting through the available information and reviewing critically the up-to-date progress in HPLC for selecting a specific analysis is available in a single book. Selection of the HPLC Method in Chemical Analysis is an inclusive go-to reference for HPLC method selection, development, and validation.

Addresses the various aspects of practice and instrumentation needed to obtain reliable HPLC analysis resultsLeads researchers to the best choice of an HPLC method from the overabundance of information existent in the fieldProvides criteria for HPLC method selection, development, and validationAuthored by world-renowned HPLC experts who have more than 60 years of combined experience in the field

1. Start of the Implementation of a New HPLC Method 2. Short Overviews of Analytical Techniques Not Containing an Independent Separation Step 3. Short Overviews of the Main Analytical Techniques Containing a Separation Step 4. Basic Information Regarding the HPLC Techniques 5. Properties of Analytes and Matrices Determining HPLC Selection 6. General Aspects Regarding the HPLC Analytical Column 7. RP-HPLC Analytical Columns 8. Polar Analytical Columns 9. Stationary Phases and Columns for Ion Exchange, Ion-Moderated, and Ligand Exchange Chromatography 10. Stationary Phases and Columns for Chiral Chromatography 11. Stationary Phases and Columns for Size Exclusion 12. Stationary Phases and Columns for Immunoaffinity Type Separations 13. Solvents, Buffers, and Additives Used in the Mobile Phase 14. Gradient Elution 15. The Practice of HPLC