Intermediate Filament Proteins

Intermediate Filament Proteins, the latest volume in the Methods in Enzymology series covers all the intermediate filaments in vertebrates and invertebrates, providing a unique understanding of the multiple different tissue-specific intermediate filaments.

This volume also covers the latest methods that are currently being used to study intermediate filament protein function and dynamics. It will be an important companion for any experimentalist interesting in studying this protein family in their cell or organism model system.

Part I:  General Methods to Study IF Proteins
1. How to Study Intermediate Filaments in Atomic Detail
Anastasia A. Chernyatina, John F. Hess, Dmytro Guzenko, John C. Voss and Sergei V. Strelkov
2. Mechanical Properties of Intermediate Filament Proteins
Elisabeth E. Charrier and Paul A. Janmey
3. Multidimensional Monitoring of Keratin Intermediate Filaments in Cultured Cells and Tissues
Nicole Schwarz, Marcin Moch, Reinhard Windoffer and Rudolf E. Leube
4. Phospho-Specific Antibody Probes of Intermediate Filament (IF) Proteins
Hidemasa Goto, Hiroki Tanaka, Kousuke Kasahara  and Masaki Inagaki
5. Assays for Post-Translational Modifications of Intermediate Filament Proteins
Natasha T. Snider and M. Bishr Omary
6. Immunofluorescence and Immunohistochemical Detection of Keratins
Cornelia Stumptner, Margit Gogg-Kamerer, Christian Viertler, Helmut Denk and Kurt Zatloukal
7. High Throughput Screening for Drugs that Modulate Intermediate Filament Proteins
Jingyuan Sun , Vincent E. Groppi , Honglian Gui, Lu Chen, Qing Xie, Li Liu and M. Bishr Omary
8. The Use of Withaferin A to Study Intermediate Filaments
Royce Mohan and Paola BargagnaMohan
9. Assays to Study Consequences of Cytoplasmic Intermediate Filament Mutations: the Case of Epidermal Keratins
Tong San Tan, Yi Zhen Ng, Cedric Badowski, Tram Dang, John E A Common, Lukas Lacina, Ildikó Szeverényi and E Birgitte Lane
10. Using Data Mining and Computational Approaches to Study Intermediate     Filament Structure and Function
David A.D. Parry
Part II: Mammalian IF Proteins
11. Isolation and Analysis of Keratins and Keratin Associated Proteins from Hair and Wool
Santanu Deb-Choudhury, Jeffrey E. Plowman and Duane P. Harland
12. Skin Keratins
Fengrong Wang, Abigail Zieman and Pierre A. Coulombe
13. Simple Epithelial Keratins
Pavel Strnad, Nurdan Guldiken, Terhi O. Helenius, Julia O. Misiorek, Joel H. Nyström, Iris A.K. Lähdeniemi, Jonas S.G. Silvander, Deniz Kuscuoglu and Diana M. Toivola
14. Methods for Determining the Cellular Functions of Vimentin Intermediate Filaments
Karen M. Ridge, Dale Shumaker, Amélie Robert, Caroline Hookway, Vladimir I. Gelfand, Paul A. Janmey, Jason Lowery, Ming Guo, David A. Weitz, Edward Kuczmarski, and Robert D. Goldman
15. Strategies to Study Desmin  in Cardiac Muscle and Culture Systems
Antigoni Diokmetzidou, Mary Tsikitis, Sofia Nikouli, Ismini Kloukina, Elsa Tsoupri, Stamatis Papathanasiou, Stelios Psarras, Manolis Mavroidis and Yassemi Capetanaki
16. Genetic Manipulation of Neurofilament Protein Phosphorylation
Maria R. Jones, Eric Villalón and Michael L. Garcia
17. α-Internexin and Peripherin: Expression, Assembly, Functions and Roles in Disease
Jian Zhao and Ronald K. H. Liem
18. Studying Nestin and its Inter-Relationship with Cdk5
Julia Lindqvist, Num Wistbacka and John E. Eriksson
19. Synemin: Molecular Features and the Use of Proximity Ligation Assay to Study its Interactions
Madhumita Paul and Omar Skalli
20. Targeting Mitogen-Activated Protein Kinase Signaling in Mouse Models of Cardiomyopathy Caused By Lamin A/C Gene Mutations
Antoine Muchir and Howard J Worman
21. In Vivo, Ex Vivo and in Vitro Approaches to Study Intermediate Filaments in the Eye Lens
M. Jarrin, L. Young, W. Wu, J.M. Girkin and R.A. Quinlan
Part III:  Non-Mammalian IF Protein Systems
22. Compartment Specific Phosphorylation of Squid Neurofilaments
Philip Grant and Harish C. Pant
23. Using Xenopus Embryos to Study Transcriptional and Post-Transcriptional Gene Regulatory Mechanisms of Intermediate Filaments
Chen Wang and Ben G. Szaro
24. Intermediate Filaments in Caenorhabditis Elegans
Noam Zuela and Yosef Gruenbaum
25. Mechanical Probing of the Intermediate Filament-Rich C. Elegans Intestine
Oliver Jahnel, Bernd Hoffmann, Rudolf Merkel, Olaf Bossinger and  Rudolf E. Leube
26. Using Drosophila for Studies of Intermediate Filaments
Jens Bohnekamp, Diane E. Cryderman, Dylan A. Thiemann, Thomas M. Magin and Lori L. Wallrath