Methods for Analysis of Carbohydrate Metabolism in Photosynthetic Organisms: Plants, Green Algae and Cyanobacteria

Methods for Analysis of Carbohydrate Metabolism in Photosynthetic Organisms: Plants, Green Algae and Cyanobacteria examines both general and detailed aspects of carbohydrate metabolism in photosynthetic organisms, along with the four main oligosaccharides and each enzymatic reaction that gives birth to them. Chapters include information on how biological active protein is extracted for different cells, determination of enzymatic activity, separation of proteins by different available methods, and descriptions of analytical methods for the determination of various types of carbohydrates in photosynthetic organisms.

The book contains useful protocols for researchers working on the determination of carbohydrate metabolism. The book provides foundational content as well as step-by-step guidance on how to design and conduct an experiment, including what other methodologies could be used if advanced instruments are not readily available.

Includes a variety of analytical methods and how to apply the methods using examples from specific case studiesDiscusses technical information on how to characterize plant carbohydrates and sugar nucleosidesContains easy-to-follow protocols with detailed explanations for self-guidanceProvides foundational content as well as step-by-step guidance on how to design and conduct an experiment

Part I. General Analytical Methods 1. Determination of carbohydrates metabolism molecules

Part II. Purification and analysis of proteins and carbohydrates 2. Preparation of protein extracts 3. Protein and carbohydrate separation and purification 4. Measurement of enzyme activity 5. General introduction to Mass Spectrometry and Nuclear Magnetic Resonance

Part III. Case studies 6. Sucrose 7. Trehalose 8. Raffinose 9. Fructans 10. Polysaccharides 11. Starch 12. Glycogen 13. Cellulose 14. Sugar phosphates 15. Nucleotide sugars