The Neuronal Cytoskeleton, Motor Proteins, and Organelle Trafficking in the Axon

The Neuronal Cytoskeleton, Motor Proteins, and Organelle Trafficking in the Axon, a new volume in the Methods in Cell Biology series continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in neuronal cells, and includes sections on such topics as actin transport in axons and neurofilament transport.

Covers an increasingly appreciated field in cell biologyIncludes both established and new technologies Contributed by experts in the field

1. Axonal Transport: The Orderly Motion of Axonal Structures
Mark M Black
2. Live-Cell Imaging of Neurofilament Transport in Cultured Neurons
Atsuko Uchida, Paula C. Monsma, J. Daniel Fenn, Anthony Brown
3. Axonal Actin in Action: Imaging Actin Dynamics in Neurons
Kelsey Ladt, Archan Ganguly, Subhojit Roy
4. Live Imaging of Microtubule Dynamics in Organotypic Hippocampal Slice Cultures
Philipp Schätzle, Lukas C. Kapitein, Casper C. Hoogenraad
5. Studying Neuronal Microtubule Organization and Microtubule-Associated Proteins Using Single Molecule Localization Microscopy
Anaël Chazeau, Eugene A. Katrukha, Casper C. Hoogenraad, Lukas C. Kapitein
6. The Use of Proteomic Analysis to Study Trafficking Defects in Axons
Xiaoqin Fu, Kristy J. Brown, Sree Rayavarapu, Kanneboyina Nagaraju, Judy S. Liu
7. Using siRNA to Study Microtubule-Related Proteins in Cultured Neurons
Lanfranco Leo, Wenqian Yu, Peter W. Baas
8. Optimization of Cerebellar Purkinje Neuron Cultures and Development of a Plasmid-Based Method for Purkinje Neuron-Specific, miRNA-Mediated Protein Knockdown
C. J. Alexander, J. A. Hammer III
9. Biochemical Analysis of Axon-Specific Phosphorylation Events Using Isolated Squid Axoplasms
Minsu Kang, Lisa Baker, Yuyu Song, Scott Brady, Gerardo Morfini
10. Analyzing Kinesin Motor Domain Translocation in Cultured Hippocampal Neurons
Rui Yang, Marvin Bentley, Chung-Fang Huang, Gary Banker
11. Imaging and Quantitative Analysis of Cytokinesis in Developing Brains of Kinesin-6 Mutant Mice
Kerstin M. Janisch, Noelle D. Dwyer
12. Live Cell Imaging of Cytoplasmic Dynein Movement in Transfected Embryonic Rat Neurons
Mitchell W. Ross, David J. Mitchell, John C. Cain, Kiev R. Blasier, K. Kevin Pfister
13. Live-Cell Imaging of Retrograde Transport Initiation in Primary Neurons
Jeffrey J. Nirschl, Erika L. F. Holzbaur
14. Methods to Identify and Analyze Gene Products Involved in Neuronal Intracellular Transport Using Drosophila
Amanda L. Neisch, Adam W. Avery, James B. Machamer, Min-gang Li, Thomas S. Hays
15. In vivo Analysis of Axonal Transport in Zebrafish
Catherine M Drerup, Alex V Nechiporuk
16. Fast Axonal Transport in Isolated Axoplasm from the Squid Giant Axon
Yuyu Song, Minsu Kang, Gerardo Morfini, Scott T. Brady
17. Cellular and Subcellular Imaging of Motor Protein-Based Behavior in Embryonic Rat Brain
Alexandre D. Baffet, Aurélie Carabalona, Tiago J. Dantas, David D. Doobin, Daniel J. Hu, Richard B. Vallee
18. Tracking Quantum-Dot Labeled Neurotropic Factors Transport Along Primary Neuronal Axons in Compartmental Microfluidic Chambers
Shani Gluska, Michael Chein, Nimrod Rotem, Ariel Ionescu, Eran Perlson
19. Studying Endosomes in Cultured Neurons by Live-Cell Imaging
Zofia M. Lasiecka, Bettina Winckler
20. Comparative Analysis of Axonal Transport Markers in Primary Mammalian Neurons
Eva Klinman, Erika L. F. Holzbaur
21. Imaging Organelle Transport in Primary Hippocampal Neurons Treated with Amyloid-ß Oligomers
Kathlyn J. Gan, Michael A. Silverman
22. High Spatial and Temporal Resolution Imaging of Motor-dependent Transport in Neuronal and Nonneuronal Cells
Julie Yi, Noopur V. Khobrekar, Tiago J. Dantas, Jie Zhou, Richard B. Vallee
23. Isolation and Analyses of Axonal Ribonucleoprotein Complexes
Ella Doron-Mandel, Stefanie Alber, Juan A. Oses, Katalin F. Medzihradszky, Alma L. Burlingame, Mike Fainzilber, Jeffery L. Twiss, Seung Joon Le