Isotope Labeling of Biomolecules – Labeling Methods

Isotope Labeling of Biomolecules – Labeling Methods, the latest volume of  the Methods in Enzymology series contains comprehensive information on stable isotope labeling methods and applications for biomolecules.

Contains contributions from leading authorities in the field of isotope labeling of biomolecules Informs and updates on the latest developments in the fieldProvides comprehensive information on stable isotope labeling methods and applications for biomolecules

Labeling in Prokarya 1. Robust High Yield Methodologies for 2H and 2H/15N/13C Labelling of Proteins for Structural Investigations using Neutron Scattering and NMR Anthony P. Duff, Karyn L. Wilde, Agata Rekas, Vanessa Lake and Peter J. Holden 2. Protein labeling in Escherichia coli with 2H, 13C and 15N J. Todd Hoopes, Margaret A. Elberson, Renae J. Preston, Prasad T. Reddy and Zvi Kelman 3. Escherichia Coli Auxotroph Host Strains for Amino Acid-Selective Isotope Labeling of Recombinant Proteins Myat T. Lin, Risako Fukazawa, Yoshiharu Miyajima-Nakano, Shinichi Matsushita, Sylvia K. Choi, Toshio Iwasaki and Robert B. Gennis 4. 19F-Modified Proteins and 19F-Containing Ligands as Tools in Solution NMR Studies of Protein Interactions Naima G. Sharaf and Angela M. Gronenborn 5. Biopolymer Deuteration For Neutron Scattering and Other Isotope-Sensitive Techniques Robert A. Russell, Christopher J. Garvey, Tamim A. Darwish, L. John R. Foster and Peter J. Holden 6. Production of Bacterial Cellulose with Controlled Deuterium-Hydrogen Substitution for Neutron Scattering Studies Hugh O’Neill, Riddhi Shah, Barbara Evans, Junhong He, Sai Venkatesh Pingali, Shishir P.S. Chundawat, A. Daniel Jones, Paul Langan, Brian H. Davison and Volker Urban 7. Isotopic Labeling of Proteins in Halobacterium Salinarum Thomas E. Cleveland and Zvi Kelman 8. Amino Acid Selective Unlabeling in Protein NMR Spectroscopy Chinmayi Prasanna, Abhinav Dubey and Hanudatta S. Atreya

Labeling in Eukarya 9. Isotope Labeling of Eukaryotic Membrane Proteins in Yeast for Solid-State NMR Ying Fan, Sanaz Emami, Rachel Munro, Vladimir Ladizhansky and Leonid S. Brown 10. Development of Approaches for Deuterium Incorporation in Plants Barbara R. Evans and Riddhi Shah 11. Isotope labeling of proteins in insect cells Lukasz Skora, Binesh Shrestha and Alvar D. Gossert 12. Effective Isotope Labeling of Proteins in a Mammalian Expression System Mallika Sastry, Carole A. Bewley and Peter D. Kwong

In Vitro Labeling 13. Escherichia Coli Cell-Free Protein Synthesis and Isotope Labeling of Mammalian Proteins Takaho Terada and Shigeyuki Yokoyama 14. Rapid Biosynthesis of Stable Isotope Labeled Peptides from a Reconstituted in Vitro Translation System for Targeted Proteomics Feng Xian, Shuwei Li and Siqi Liu 15. Labeling of Membrane Proteins by Cell-Free Expression Aisha Laguerre, Frank Löhr, Frank Bernhard and Volker Dötsch 16. Selective Amino Acid Segmental Labeling of Multi-Domain Proteins Erich Michel and Frédéric H.-T. Allain 17. Labeling Monosaccharides with Stable Isotopes Wenhui Zhang, Shikai Zhao and Anthony S. Serianni