Analytical Ultracentrifugation

Analytical Ultracentrifugation, the latest volume in Methods in Enzymology, focuses on analytical ultracentrifugation. The scope of this technique has greatly expanded in recent years due to advances in instrumentation, algorithms and software.

This volume describes the latest innovations in the field and in the applications of analytical ultracentrifugation in the analysis of macromolecules, macromolecular assemblies, and biopharmaceuticals.

1. Next Generation AUC adds a Spectral Dimension: Development of Multiwavelength Detectors for the Analytical Ultracentrifuge
Joseph Pearson, Frank Krause, Dirk Haffke, Borries Demeler, Kristian Schilling and Helmut Cölfen
2. Next Generation AUC: Analysis of Multi-Wavelength Analytical Ultracentrifugation Data
Gary E. Gorbet, Joseph Z. Pearson, Aysha K. Demeler, Helmut Cölfen and Borries Demeler
3. Sedimentation Velocity: A Classical Perspective
John J. Correia and Walter F. Stafford
4. Hydrodynamic Modeling and its Application in AUC
Mattia Rocco and Olwyn Byron
5. Calculations and Publication-Quality Illustrations for Analytical Ultracentrifugation Data
Chad A. Brautigam
6. Sedimentation Equilibrium Analysis of Clpb Self-Association in Diluted and Crowded Solutions.
Carlos Alfonso, Urko del Castillo, Ianire Martín, Arturo Muga and Germán Rivas
7. Analysis of Linked Equilibria
JiaBei Lin and Aaron L. Lucius
8. Elucidating Complicated Assembling Systems in Biology Using Size-And-Shape Analysis of Sedimentation Velocity Data
Catherine T. Chaton and Andrew B. Herr
9. Quaternary Structure Analyses of an Essential Oligomeric Enzyme
Tatiana P. Soares da Costa, Janni B. Christensen, Sebastien Desbois, Shane E. Gordon, Ruchi Gupta, Campbell J. Hogan, Tao G. Nelson, Matthew T. Downton, Chamodi K. Gardhi, Belinda M. Abbott, John Wagner, Santosh Panjikar and Matthew A. Perugini
10. Characterization of Intrinsically Disordered Proteins by Analytical Ultracentrifugation
David J. Scott and Donald J. Winzor
11. Sedimentation Velocity Analysis of the Size Distribution of Amyloid Oligomers and Fibrils
Yee-Foong Mok, Geoffrey J. Howlett and Michael D.W. Griffin
12. AUC and Small Angle Scattering for Membrane Proteins
Aline Le Roy, Kai Wang, Béatrice Schaack, Peter Schuck, Cécile Breyton and Christine Ebel
13. Hydrodynamic Models of G-quadruplex Structures
Jonathan B. Chaires, William L. Dean, Huy Le and John O. Trent
14. Analytical Ultracentrifugation as a Tool to Study Non-specific Protein-DNA Interactions
Teng-Chieh Yang, Carlos Enrique Catalano and Nasib Karl Maluf
15. Characterization of Homogeneous, Cooperative Protein-DNA Clusters by Sedimentation Equilibrium Analytical Ultracentrifugation and Atomic Force Microscopy
Ingrid Tessmer and Michael G. Fried
16. Sedimentation Velocity Analysis of Large Oligomeric Chromatin Complexes Using Interference Detection.
Hansen and Jeffrey
17. Methods in Enzymology – Analytical Ultracentrifugation
David L Bain, Rolando W De Angelis, Keith D Connaghan, Qin Yang, Gregory D Degala and James R Lambert
18. Ultracentrifuge Methods for the Analysis of Polysaccharides, Glycoconjugates and Lignins
Stephen E. Harding, Gary G. Adams, Fahad Almutairi, Qushmua Alzahrani, Tayyibe Erten, M. Samil Kök and Richard B. Gillis
19. Analytical Ultracentrifugation and its Role in Development and Research of Therapeutical Proteins.
Jun Liu, Sandeep Yadav, James Andya, Barthélemy Demeule and Steven J. Shire
20. Guidance to Achieve Accurate Aggregate Quantitation in Biopharmaceuticals by SV-AUC
Kelly K. Arthur, Brent S. Kendrick and John P. Gabrielson
21. Protein Assembly in Serum and the Differences from Assembly in Buffer.
John J. Hill and Thomas M. Laue