Calorimetry

Calorimetry, the latest volume in the Methods in Enzymology series continues the legacy of this premier serial with quality chapters authored by leaders in the field. Calorimetry is a highly technical experiment and it is easy for new practioners to get fooled into interpreting artifacts as real experimental results.  This volume will guide readers to get the most out of their precious biological samples and includes topics on specific protocols for the types of studies being conducted as well as tips to improve the data collection. Most importantly, the chapters will also help to identify pitfalls that need to be avoided to ensure that the highest quality results are obtained.

Contains timely contributions from recognized experts in this  rapidly changing fieldProvides specific protocols and tips to improve data collection and ensure the highest quality results are obtained Covers research methods in calorimetry, and includes sections on topics such as differential scanning calorimetry of membrane and soluble proteins in detergents.

1. Isothermal Titration Calorimetry Measurements of Metal Ions Binding to Proteins
Colette F. Quinn ,Margaret C. Carpenter, Molly L. Croteau and Dean E. Wilcox
2. Assessing Coupled Protein Folding and Binding through Temperature- Dependent    Isothermal Titration Calorimetry
Debashish Sahu, Monique Bastidas, Chad W. Lawrence, William G. Noid,and Scott  A. Showalter
3. Fragment-Based Screening for Enzyme Inhibitors Using Calorimetry
Michael I. Recht, Vicki Nienaber and Francisco E. Torres
4. Measuring Multivalent Binding Interactions by Isothermal Titration Calorimetry
Tarun K. Dam,  Melanie L Talaga, Ni Fan and Curtis F. Brewer
5. Calorimetric and Spectroscopic Analysis of the Thermal Stability of Short Duplex DNA-Containing Sugar and Base-Modified Nucleotides
Kareem Fakhfakh, Curtis B. Hughesman, A. Louise Creagh, Vincent Kao and  Charles Haynes
6. Calorimetric Quantification of Cyclodextrin-Mediated Detergent Extraction for Membrane-Protein Reconstitution
Martin Textor and Sandro Keller
7. Extending ITC to Kinetics with KinITC
Philippe Dumas, Eric Ennifar, Cyrielle Da Veiga, Guillaume Bec, William Palau, Carmelo Di Primo,Angel Piñeiro, Juan Sabin, Eva Muñoz and Javier Rial
8. Measuring the Kinetics of Molecular Association by Isothermal Titration Calorimetry
Kirk A. Vander Meulen, Scott Horowitz, Raymond C. Trievel, and Samuel E. Butcher
9. Isothermal Titration Calorimetry to Characterize Enzymatic Reactions
Luca Mazzei, Stefano Ciurli and Barbara Zambelli
10. Avoiding Buffer Interference in Itc Experiments: A Case Study from the Analysis of  Entropy Driven Reactions of Glucose-6-Phosphate Dehydrogenase
M. Lucia Bianconi
11. ITC Methods for Assessing Buffer/Protein Interactions from the Perturbation of  Steady-State Kinetics: A Reactivity Study of Homoprotocatechuate 2,3-Dioxygenase
Kate L. Henderson, Delta K. Boyles, Vu H. Le, Edwin A. Lewis and Joseph P.Emerson
12. Modern Analysis of Protein Folding by Differential Scanning Calorimetry
Beatriz Ibarra-Molero, Athi N. Naganathan, Jose M. Sanchez-Ruiz and Victor Muñoz
13. A Guide to Differential Scanning Calorimetry of Membrane and Soluble Proteins in Detergents
Zhengrong Yang and Christie G. Brouillette
14. Orthogonal Methods for Characterizing the Unfolding of Therapeutic Monoclonal  Antibodies: Differential Scanning Calorimetry, Isothermal Chemical Denaturation and  Intrinsic Fluorescence with Concomitant Static Light Scattering
Deniz B. Temel, Pavel Landsman and Mark L. Brader
15. IATC, DSC, and PPC Analysis of Reversible and Multi-State Structural Transition of Cytochrome c
Shun-ichi Kidokoro and Shigeyoshi Nakamura
16. The Complementarity of the Loop to the Stem in DNA Pseudoknots Gives Rise to Local TAT Base-Triplets
Calliste Reiling-Steffensmeier and Luis A. Marky
17. A High-Throughput Biological Calorimetry Core: Steps to Startup, Run, and Maintain a Multi-User Facility
Neela H. Yennawar, Julia A. Fecko, Scott A. Showalter and Philip C. Bevilacqua