Diagnostic Molecular Biology

Diagnostic Molecular Biology describes the fundamentals of molecular biology in a clear, concise manner to help readers comprehend this complex subject. Each technique is explained within a conceptual framework to enhance understanding, and current applications of clinical laboratory techniques are covered in detail. This targeted approach covers the principles of molecular biology, including the basic knowledge of nucleic acids, proteins, chromosomes, and the basic techniques and instrumentations that are commonly used in the field of molecular biology. In addition, detailed procedures and explanations, and the applications of the principle and techniques currently employed in the clinical laboratory are covered.

Provides an understanding of what techniques are used to diagnosis at the molecular levelExplains how to use information technology to communicate and assess results in the labPlaces protocols into context with practical applications

Part I 1. Nucleic Acids: DNA and RNA 2. Nucleic Acid-Based Cellular Activities 3. Gene Expression: Transcription of the Genetic Code 4. Gene Expression: Translation of the Genetic Code 5. The Genome

Part II 6. Extraction and Purification of Nucleic Acids and Proteins 7. Detection and Analysis of Nucleic Acids 8. Quantification and Analysis of Proteins 9. Amplification of Nucleic Acids 10. Characterization of Nucleic Acids and Proteins 11. Techniques in Sequencing 12. Genome and Transcriptome Analysis

Part III 13. Molecular Diagnosis of Chromosomal Disorders 14. Molecular Diagnosis of Mutation and Inherited Diseases 15. Molecular Diagnosis of Infectious Diseases 16. Guidance for Molecular Clinical Laboratory