Membrane Proteins – Production and Functional Characterization

Membrane Proteins – Production and Function Characterization a volume of Methods in Enzymology, encompasses chapters from the leading experts in the area of membrane protein biology. The chapters provide a brief overview of the topics covered and also outline step-by-step protocol. Illustrations and case example images are included wherever appropriate to help the readers understand the schematics and general experimental outlines.

Volume of Methods In EnzymologyContains a collection of a diverse array of topics in the area of membrane protein biology ranging from recombinant expression, isolation, functional characterization, biophysical studies and crystallization

Section I: Recombinant Expression of Membrane Proteins
1. Engineering Escherichia Coli for Functional Expression of Membrane Proteins
Franz Y Ho and Bert Poolman
2. ACEMBLing a Multiprotein Transmembrane Complex:  The Functional SecYEG-SecDFYajC-YidC  Holotranslocon Protein Secretase/Insertase
Joanna Komar, Mathieu Botte, Ian Collinson, Christiane Schaffitzel and Imre Berger
3.  Expression and Purification of OsVDAC4
Ashwini Godbole, Ashvini K. Dubey, Rohan Mitra, Palakolanu S. Reddy and M. K. Mathew
4. Membrane Protein Expression in Lactococcus Lactis
Martin S. King, Christoph Boes and Edmund R.S. Kunji
5. An Unconventional Anaerobic Membrane Protein Production System Based on Wolinella Succinogenes
Michael Lafontaine and C. Roy D. Lancaster
6. Membrane Protein Expression and Analysis in Yeast
Katrien Claes, Mouna Guerfal and Nico Callewaert
7. Heterologous Expression of G Protein Coupled Receptors in Yeast
Nicolas Bertheleme, Shweta Singh, Simon Dowell and Bernadette Byrne
8. Recombinant G protein-Coupled Receptor Expression in Saccharomyces Cerevisiae for Protein Characterization
Kory M. Blocker, Zachary T. Britton, Andrea N. Naranjo, Patrick M. McNeely, Carissa L. Young and Anne S. Robinson
9. Baculovirus Mediated Expression of GPCRs in Insect Cells
Tuulia Saarenpää, Veli-Pekka Jaakola and Adrian Goldman
10. Expression of Membrane Proteins in the Eyes of Transgenic Drosophila Melanogaster
Yvonne Hackmann, Lisa Joedicke, Valérie Panneels and Irmgard Sinning
11. Rapid Method to Express and Purify Human Membrane Protein Using the Xenopus Oocyte System for Functional and Low-Resolution Structural Analysis
Benjamin Clémençon, Michael Fine, Philipp Schneider and Matthias A. Hediger
12. Expression of G protein-coupled Receptors in Mammalian Cells
Raja Chakraborty, Bing Xu, Rajinder P. Bhullar and Prashen Chelikani
13. Construction of Stable Mammalian Cell Lines for Inducible Expression of G Protein-Coupled Receptors
Chikwado A. Opefi, Dale Tranter, Steven O. Smith and Philip J. Reeves.
14. Rapid and Facile Recombinant Expression of Bovine Rhodopsin in HEK293S GnTI– Cells Using a PiggyBac Inducible System
Lydia N. Caro, Zhijie Li, James M. Rini, Aidin R. Balo and Oliver P. Ernst
15. Semliki Forest Virus-based Expression of Recombinant GPCRs
Kenneth Lundstrom
16. Screening for Lipid Requirements of Membrane Proteins by Combining Cell-Free Expression with Nanodiscs
Erik Henrich, Volker Dötsch, and Frank Bernhard
Section II. Reconstitution and Functional Characterization of Membrane Proteins
17. Liposome Reconstitution and Transport Assay for Recombinant Transporters
Zachary Lee Johnson and Seok-Yong Lee
18. Lipid Reconstitution and Recording of Recombinant Ion Channels
Randy Stockbridge and Ming-Feng Tsai
19. Reconstitution of Membrane Proteins - a GPCR as an Example
Alan D. Goddard, Patricia M. Dijkman, Roslin J. Adamson, Rosana Inácio dos Reis and Anthony Watts
20. Ion Channel Reporter for Monitoring the Activity of Engineered GPCRs
Christophe J. Moreau,  Katarzyna Niescierowicz , Lydia Caro , Jean Revilloud and  Michel Vivaudou
21. Methods for Labeling Skeletal Muscle Ion Channels Site-Specifically With Fluorophores Suitable for FRET-based Structural Analysis
Mohana Mahalingam and James D. Fessenden
22. Structural and Functional Studies of Nirc From Salmonella Typhimurium
Adriana Rycovska-Blume,Wei Lü, Susana Andrade, Klaus Fendler and Oliver Einsle
23. Surface Plasmon Resonance Analysis of 7-Transmembrane Receptors
Tonia Aristotelous, Andrew L. Hopkins and Iva Navratilova
24. Crosslinking Strategies to Study Peptide Ligand-Receptor Interactions
Jeffery Becker
25. From recombinant expression to crystals – A step-by-step guide to GPCR crystallography
 Arun K. Shukla, Punita Kumari, Eshan Ghosh and Kumari Nidhi
26. Structure-based Biophysical Analysis of the Interaction of Rhodopsin with G Protein and Arrestin
Martha E. Sommer, Matthias Elgeti, Peter W. Hildebrand, Michal Szczepek, Klaus Peter Hofmann and Patrick Scheerer