Lysosomes and Lysosomal Diseases

This new volume of Methods in Cell Biology looks at methods for lysosomes and lysosomal diseases.  Chapters focus upon practical experimental protocols to guide researchers through the analysis of multiple aspects of lysosome biology and function. In addition, it details protocols relevant to clinical monitoring of patients with lysosomal diseases. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come.

Covers sections on model systems and functional studies, imaging-based approaches and emerging studiesChapters are written by experts in the fieldCutting-edge material

1. Methods for Monitoring Lysosomal Morphology
Bethan S. Kilpatrick, Emily R. Eden, Leanne N. Hockey, Clare E. Futter and Sandip Patel
2. A Rapid Method for the Preparation of Ultra-pure, Functional Lysosomes Using Funcionalised Superparamagnetic Iron Oxide Nanoparticles
Mathew William Walker and Emyr Lloyd-Evans
3. TFEB and the CLEAR Network
Carmine Settembre and Diego L. Medina
4. Biosynthesis, Targeting and Processing of Lysosomal Proteins: Pulse-Chase Labeling and Immune Precipitation
Sandra Pohl and Andrej Hasilik
5. Measuring Lysosomal pH by Fluorescence Microscopy
Johnathan Canton and Sergio Grinstein
6. Lysosome Fusion in Cultured Mammalian Cells
Nicholas A. Bright, Lena Wartosch and J. Paul Luzio
7. RNAi Screens of Lysosomal Trafficking
Salil Garg, Michael B. Brenner
8. Approaches for Plasma Membrane Wounding and Assessment of Lysosome-mediated Repair Responses
Corrotte, M, Castro-Gomes, T, Koushik, A.B. and Andrews, N.W
9. Imaging Approaches to Measuring Lysosomal Calcium
Anthony J. Morgan, Lianne C. Davis, Antony Galione
10. Lysosome Electrophysiology
Xi Zoë Zhong and Xian-Ping Dong
11. Reconstitution of Lysosomal Ion Channels into Artificial Membranes
Elisa Venturi and Rebecca Sitsapesan
12. Fluorescence Methods for Analysis of Interactions Between Ca2+ Signalling, Lysosomes and Endoplasmic Reticulum
David L. Prole, Cristina I. López-Sanjurjo, Stephen C. Tovey and Colin W. Taylor
13. Methods for the Quantification of Lysosomal Membrane Permeabilization: a Hallmark of Lysosomal Cell Death
Sonja Aits, Marja Jäättelä and Jesper Nylandsted
14. Measuring the Phagocytic Activity of Cells
Nick Platt and Paul Fineran
15. Detection and Quantification of Microbial Manipulation of Phagosomal Function
Maria Podinovskaia, David G. Russell
16. Measuring Relative Lysosomal Volume for Monitoring Lysosomal Storage Diseases
Danielle te Vruchte, Kerri L. Wallom and Frances M. Platt
17. Quantifying Storage Material Accumulation in Tissue Sections
Jonathan D Cooper, Helen R Brooks, Hemanth R Nelvagal
18. Laboratory Diagnosis of Niemann-Pick disease Type C: The Filipin Staining Test
Marie T. Vanier and Philippe Latour