Video Microscopy

Video microscopy is used extensively in many life and biomedical science disciplines today, and is a useful tool for both cell biologists and students. This book presents how to track the dynamic changes that take place in the structure of living cells and in reconstituted preparations using video and digital imaging microscopy. Basic information, principles, and applications are also covered, as well as more specialized video microscopy techniques.

Chapters cover the commonly used video technologies in biological researchNontechnical presentation of principlesEmphasis on the practical aspects of instrument useCovers pitfalls in instrument uses that can lead to artifactsAuthors are leaders in the design and application of video methods to biological microscopyPresentation of material tailored for the established researcher that has little experience with video methodsExamples are extensively illustrated with photographs

G. Sluder and E.H. Hinchcliffe, Video Basics: Use of Camera and Monitor Adjustments. K.A. Jacobsen and K. Berland, Electronic Cameras for Low Light Microscopy. M. Oshiro, Cooled CCD vs Intensified Cameras forLow Light Video--Applications and Relative Advantages. T. Inoue and N. Gliksman, Techniques for Optimizing Microscopy and Analysis Through Digital Image Processing. R. Cardullo, Basics of Image Processing: Analog and Digital. D. Wolf, Quantitative Video Microscopy. E. Keller, Proper Alignment of the Microscope. J. Hinsch, Mating Cameras to Microscopes. E.D. Salmon and P. Tran, High Resolution Video-Enhanced Differential Interference Contrast (VE-DIC) Light Microscopy. E.D. Salmon, S.L. Shaw, J. Waters, C.M. Waterman-Storer, P.S. Maddox, E. Yeh, and K. Bloom A High-Resolution Multi-Mode Digital Microscope System. F.R. Maxfield and K. Dunn, Ratio Imaging Instrumentation. R.B. Silver, Ratio Imaging: Practical Considerations for Measuring Intracellular Calcium and pH in Living Tissue. C. Rieder and R.W. Cole, Perfusion Chambers for High Resolution Video Light Microscopic Studies of Vertebrate Cell Monolayers: Some Considerations and a Design. E. Gratton, P.T.C. So, C.Y. Dong, T. French, and K. Berland, Fluorescence Lifetime Imaging Techniques for Microscopy. Y.-L. Wang, Digital Deconvolution of Fluorescence Images for Biologists. Subject Index.