A Practical Guide to the Study of Calcium in Living Cells

A Practical Guide to the Study of Calcium in Living Cells describes popular techniques along with helpful do's and don't's and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research needs. This book is designed for laboratory use by graduate students, technicians, and researchers in many disciplines, ranging from molecular to cellular levels of investigation.

Describes techniques for detection of [Ca2+]I: Ca2+ - sensitive microelectrodesFluorescent dyesLuminescent proteinsIncludes techniques for perturbing intracellular Ca2+Covers detailed methodology plus problems and pitfalls of each techniqueContains a practical guide to preparing Ca2+ buffers with an easy-to-use computer programColor plates illustrate techniques such asConfocal ratio-imagingUse of aequorin

Ca²⁺ Buffers and [Ca²⁺]I Perturbation Techniques:
D.M. Bers, C.W. Patton, and R. Nuccitelli, A Practical Guide to the Preparation of Ca²⁺ Buffers.
R. Zucker, Photorelease Techniques for Raising or Lowering Intracellular Ca²⁺.
D. Thomas and M.R. Hanley, Pharmacological Tools for Perturbing Intracellular Calcium Storage.
Microelectrode Techniques for Measuring [Ca²⁺]I and Ca²⁺ Fluxes:
S. Baudet, L. Hove-Madsen, and D.M. Bers, How to Make and to Use Calcium-Specific Mini- and Microelectrodes.
P.J.S. Smith, R.H. Sanger, and L.F. Jaffe, The Vibrating Ca²⁺ Electrode: A New Technique for Detecting Plasma Membrane Regions of Ca²⁺ Influx and Efflux.
C.A. Leech and G.G. Holz, IV, Application of Patch Clamp Methods to the Study of Calcium Currents and Calcium Channels.
Fluorescence Techniques for Imaging [Ca²⁺]I:
J.P.Y. Kao, Practical Aspects of Measuring [Ca²⁺] with Fluorescent Indicators.
S.J. Morris, T.B. Wiegmann, L.W. Welling, and B.M. Chronwall, Rapid Simultaneous Estimation of Intracellular Calcium and pH.
T.J. Keating and R.J. Cork, Improved Spatial Resolution in Ratio Images Using Computational Confocal Techniques.
P.A. Diliberto, X.F. Wang, and B. Herman, Confocal Imaging of Ca²⁺ in Cells.
S. Girard and D.E. Clapham, Simultaneous Near Ultraviolet and Visible Excitation Confocal Microscopy of Calcium Transients in Xenopus Oocytes.
Use of Aequorin for [Ca²⁺]I Imaging:
K.R. Robinson, T.J. Keating, and R. J. Cork, Inexpensive Techniques for Measuring [Ca²+]I Changes Using a Photomultiplier Tube.
A.L. Miller, E. Karplus, and L.F. Jaffe, Imaging [Ca²⁺]I with Aequorin Using a Photon Imaging Detector.
R. Rizzuto, M. Brini, and T. Pozzan, Targeting Recombinant Aequorin to Specific Intracellular Organelles.
Index.