Caenorhabditis Elegans: Molecular Genetics and Development

An updated edition of the classic Methods in Cell Biology volume 48, this book emphasizes diverse methods and technologies needed to investigate C. elegans, both as an integrated organism and as a model system for research inquiries in cell, developmental, and molecular biology, as well as in genetics and pharmacology. By directing its audience to tried-and-true and cutting-edge recipes for research, this comprehensive collection is intended to guide investigators of C. elegans for years to come.

Diverse, up-to-date techniques covered will be useful to the broadening community of C. elegans researchers for years to comeChapters written by leaders in the fieldTried and true methods deliver busy researchers a one-stop compendium of essential protocols

Culturing methods and genetic procedures General methods of culture: Jim Lewis Mutagenesis: Phil Anderson Genetic screening methods: James Thomas, Eric Jorgensen, Eric Lambie Genetic mapping methods: Ben Williams, Stephen Wicks (Boston College) Genetic mosaic analysis: Robert Herman Use of specialized chromosomes and rearrangements: Ann Rose, David Baillie Gene knockouts: Shohei Mitani, Robert Barstead RNAi and RNAi screening methods: Julie Ahringer, Andrew Dillin

Genomics and Bioinformatics Transcriptional profiling: Stuart Kim, Don Moerman, Valerie Reinke MicroRNA methods: Victor Ambros, Frank Slack, Amy Pasquinelli Analysis of RNA function: Marv Wickens, Judith Kimble Analysis of the interactome: Marc Vidal WormBase: Eric Schwartz, Paul Sternberg, Lincoln Stein Structural genomics: Ming Luo, Larry DeLucas, Songlin Li, Mike Carson (U. Alabama) Proteomics: Ahna Skop, Moerman D., Isobe T, Krijgsveld, J Transcriptional regulatory networks: Marion Walhout

Markers for gene expression and protein localization Immunofluorescence and antibodies: David Miller Imaging: John White, William Mohler Electron Microscopy: David Hall Proteins and protein assemblies: Henry Epstein Analysis of morphogenesis: Jeff Hardin, Andrew Chisholm Cell death: Monica Driscoll, Ding Xue