Forced-Flow Layer Chromatography

Forced-Flow Layer Chromatography takes a close look at the specifics of forced-flow layer chromatography techniques, from their evolution to the nuances of using these techniques in a variety of applications where traditional thin-layer chromatography (TLC) and high-performance thin-layer chromatography (HPTLC) are not as effective.

This book presents a number of variations of TLC techniques, with special emphasis on the overpressured-layer chromatography (OPLC) technique and newer developments such as the BioArena System for biomedical analysis. The versatility of these forced-flow techniques opens up new avenues for the analysis of a large number of samples for high-throughput screening and for the analysis of very complex matrices, while the development of BioArena extends the use of these techniques to challenging new areas of bioanalysis.

Details a variety of forced-flow techniques, explaining how they markedly reduce developing time and result in less lateral diffusion and more compact spotsEmphasizes the benefits of OPLC separation techniques, a method pioneered by the authors nearly forty years agoDiscusses new developments, such as the BioArena system used to facilitate detection, isolation, and identification of new antimicrobials, antineoplastics, biopesticides, and other biologically active substances

1. Unique potentialities of layer liquid system - results, limitations, new demands Ernő Tyihák 2. Overpressured-layer chromatography Ernő Tyihák, Ágnes M. Móricz, and Emil Mincsovics 3. Centrifugal layer chromatography - Rotation planar chromatography Ágnes M. Móricz and Irena Vovk 4. Planar chromatography using electroosmotic flow Aneta Hałka-Grysińska, Radosław Ł. Gwarda, Tadeusz H. Dzido 5. Layer dielectrochromatography - under external alternating current, LDEC Virginia Coman and Ştefan Kreibik 6. Conventional and modern bioassays - detection, isolation, identification Ágnes M. Móricz and Péter G. Ott 7. BioArena system for studying key molecules as well as ingredients in biological samples Ernő Tyihák and Ágnes M. Móricz 8. Determining progress directions in layer liquid chromatography - dreams and realities Ernő Tyihák