Laboratory Methods in Enzymology: Protein Part C

In this volume we have brought together a number of core protocols concentrating on Protein, carefully written and edited by experts.

TCA Precipitation Coimmunoprecipitation of Proteins from Yeast Coupling Antibody to Cyanogen Bromide-Activated Sepharose Analysis of Protein–Protein Interactions by Coimmunoprecipitation Use and Application of Hydrophobic Interaction Chromatography for Protein Purification Hydroxyapatite Chromatography: Purification Strategies for Recombinant Proteins Salting out of Proteins Using Ammonium Sulfate Precipitation Using Ion Exchange Chromatography to Purify a Recombinantly Expressed Protein Gel Filtration Chromatography (Size Exclusion Chromatography) of Proteins Expression and Purification of Membrane Proteins Explanatory Chapter: Choosing the Right Detergent One-dimensional SDS-Polyacrylamide Gel Electrophoresis (1D SDS-PAGE) Coomassie Blue Staining Silver Staining of SDS-polyacrylamide Gel Standard In Vitro Assays for Protein–Nucleic Acid Interactions – Gel shift Assays for RNA and DNA Binding Protein Filter Binding Explanatory Chapter: Troubleshooting recombinant protein expression: general Explanatory Chapter: Troubleshooting protein expression: what to do when the protein is not soluble Western Blotting using Chemiluminescent Substrates