Laboratory Methods in Enzymology: RNA

Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols.

In this volume, we have brought together a number of core protocols concentrating on RNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes.

Computational Prediction of RNA Secondary Structure

Reverse-transcription PCR (RT-PCR)

Northern blotting

Explanatory Chapter: Nuclease Protection Assays

In Vitro Transcription from Plasmid or PCR-amplified DNA

Measurement of In Vivo RNA Synthesis Rates

Method for measuring mRNA decay rate in Saccharomyces cerevisiae

Analysis of polysomes from bacteria

Polysome Profile Analysis – Yeast

Polysome analysis of mammalian cells

Polysome analysis for determining mRNA and ribosome association in Saccharomyces cerevisiae

Oligo(dT)-primed RT-PCR isolation of polyadenylated RNA degradation intermediates

Circularized RT-PCR (cRT-PCR): analysis of the 5′ ends, 3′; ends, and poly(A) tails of RNA

RNA Radiolabeling

Fluorescently Labeling Synthetic RNAs

Analysis of RNA by analytical polyacrylamide gel electrophoresis

RNA purification by preparative polyacrylamide gel electrophoresis

Explanatory Chapter: Nucleic Acid Concentration Determination

RNA purification – precipitation methods

Reverse Transcriptase Dideoxy Sequencing of RNA

RNA Structure Experimental Analysis – Chemical Modification

Structural Analysis of RNA Backbone Using In-Line Probing