Methods for Analysis of Golgi Complex Function

This new volume of Methods in Cell Biology looks at methods for analyzing of golgi complex function. Chapters cover such topics as in vitro reconstitution systems, fluorescence-based analysis of trafficking in mammalian cells and high content screening. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come.

Model systems and functional studies 1.Analysis of Golgi Complex Functions: In vitro Reconstitution Systems 2.RNA Interference Approaches to Examine Golgi Function in Animal Cell Culture 3.Trafficking along the Secretory Pathway in Drosophila Cell Line and Tissues:  A Light and Electron Microscopy Approach 4.Studying Membrane Trafficking in the Worm C. elegans by RNA Interference 5.Endomembrane and Golgi Traffic in Plant Cells 6.Biochemical Analysis of Secretory Trafficking in Mammalian Cells 7.Methods for Analysis of Apical Lumen Trafficking Using Micropatterned 3D Systems 8.Using Replication Defective Viruses to Analyze Membrane Trafficking in Polarized Epithelial Cells 9.Snap-Tagging the Retrograde Route 10.Assessing ER and Golgi N-Glycosylation Process using Metabolic Labeling in Mammalian Cultured Cells

Imaging based approaches 11.Fluorescence based Analysis of Trafficking in Mammalian Cells 12.Photobleaching Methods to study Golgi Complex Dynamics in Living Cells 13.Photohighlighting approaches to access membrane dynamics of the Golgi apparatus 14.Live Cell Visualization of Golgi Membrane Dynamics by Super-Resolution Confocal Live Imaging Microscopy (SCLIM) 15.Analysis of Golgi Complex Function using Correlative Light-Electron Microscopy (CLEM) 16.Viewing Golgi Structure and Function from a different perspective – Insights from Electron Tomography 17.High Content Screening and Analysis of the Golgi Complex

Emerging studies [New Frontiers] 18.(Re)Modeling the Golgi 19.Golgi Depletion from Living Cells with Laser Nanosurgery 20.Studying Intracellular Trafficking Pathways with Probabilistic Density Maps 21.Methods to Study Signaling at The Golgi Apparatus 22.Golgi-Dependent Signaling: Self-Coordination of Membrane Trafficking 23.Signaling at the Golgi during Mitosis 24.Ice Recovery Assay for Detection of Golgi-Derived Microtubules