Methods of Adipose Tissue Biology Part A

Methods of Adipose Tissue Biology is a must-have for anyone interested in obesity or the physiology of white or brown adipose tissues. It contains state-of-the-art methods from researchers who are world leaders in this field. Detailed lab protocols include methods to visualize adipocytes and adipose tissues in humans and experimental models, converting stem cells into white and brown adipocytes in vitro, evaluating aspects of adipocyte metabolism, inducibly knocking out genes in adipose tissues, and evaluating transcriptional control of adipogenesis on a global scale.

The study of adipose tissue goes hand in hand with our global effort to understand and reverse the epidemic of obesity and associated medical complicationsContributors include leading researchers who have made tremendous contributions to our ability to investigate white and brown adipose tissuesThe wide variety of experimental approaches detailed within this volume: including the evaluation of adipose tissue biology at the molecular, biochemical, cellular, tissue, and organismal levels

1. Adipocyte-Specific Transgenic and Knockout Models
2. Imaging White Adipose Tissue With Confocal Microscopy
3. Isolation and Study of Adipocyte Precursors
4. Imaging of Adipose Tissue
5. Adipose Tissue Angiogenesis Assay
6. Quantifying Size and Number of Adipocytes in Adipose Tissue
7. Use of Osmium Tetroxide Staining with Micro-Computerized Tomography to Visualize and Quantify Bone Marrow Adipose Tissue in Vivo
8. Brown Adipose Tissue in Humans: Detection and Functional Analysis Using PET (Positron Emission Tomography), MRI (Magnetic Resonance Imaging), and DECT (Dual Energy Computed Tomography)
9. Analysing the Functions and Structure of the Human Lipodystrophy Protein Seipin
10. Differentiation of Human Pluripotent Stem Cells into Highly Functional Classical Brown Adipocytes
11. Analysis and Measurement of the Sympathetic and Sensory Innervation of White and Brown Adipose Tissue
12. Measurement and manipulation of Human Adipose Tissue Blood Flow Using Xenon Washout Technique and Adipose Tissue Microinfusion
13. Isolation and Quantitation of Adiponectin Higher Order Complexes
14. Genome-Wide Profiling of Transcription Factor Binding and Epigenetic Marks in Adipocytes by ChIP-seq
15. Analysis and Isolation of Adipocytes by Flow Cytometry
16. Flow Cytometry Analyses of Adipose Tissue Macrophages