Immunology of Infection

Immunology of Infection, 2nd Edition, edited by two leading experts in the field, presents the most appropriate up-to-date experimental approaches in the detail required for modern microbiological research. Focusing on the methods most useful for the Microbiologist interested in analysing host-pathogen relationships, this volume will be essential reading for all researchers working in microbiology, immunology, virology, mycology and parasitology.

This new edition of Immunology of Infection provides ready-to-use "recipes", and the latest emerging techniques as well as novel approaches to the tried and tested, established methods included in the successful first edition.

Methods in Microbiologyis the most prestigious series devoted to techniques and methodology in the field. Established for over 30 years, Methods in Microbiology will continue to provide you with tried and tested, cutting edge protocols to directly benefit your research.

Includes techniques for genome-wide expression profiling of both the pathogen and host and of the host response to infectionCytometric analysis of cytokine secretion by immune cells Describes tetramer technology for the quantitative analysis of antigen specific T cell responsesAnalysis of host cells and pathogens involved in the host-microbe interplay Covers techniques useful for the analysis of human and murine systems Includes techniques for the prediction and determination of MHC ligands and T cell epitopes Covers the fundamentals and practice of DNA vaccines  Describes methods for the isolation and propagation of human dendritic cells

Introduction
The immune response to infectious agents

General Methods
Phenotyping and separation of leukocyte populations based on affinity labelling
Cytometric cytokine secretion assay: detection and isolation of cytokine secreting T cells
Measurement of cellular proliferation
Use of bioinformatics to predict MHC ligands and T-cell epitopes: application to epitope driven vaccine design
The use of tetramers in the quantitative analysis of T cell responses
Using microarrays for studying the host transcriptional response to microbial infection
Genome-wide expression profiling of intracelllular bacteria: the interaction of Mycobacterium tuberculosis with macrophages

Murine Models
Management of immuno-compromised and infected animals
In Vitro Analysis
Isolation and preparation of lymphocytes from infected animals for in vitro analysis
Isolation and development of murine T-lymphocyte hybridomas and clonal cell lines
Killer cell assays
Quantitation of T Cell Cytokine Responses by ELISA, ELISPOT, Flow Cytometry and Reverse Transcriptase-PCR Methods
Isolation of and measuring the function of professional phagocytes: murine macrophages
In vivo Analysis
The immune response in mice challenged with bacterial infections
Measuring immune responses in vivo
Specific Models
Murine model of tuberculosis
The leishmaniasis model
Animal models: murine cytomegalovirus
DNA vaccines: fundamentals and practice
Preparation and use of adjuvants

Human Systems
Isolation and propagation of human dendritic cells
Isolation of T cells and establishment of T-cell lines and clones
Growth-transformation of human T cells
Generation and characterisation of human killer cells
Measuring human cytokine responses
Measuring immune responses in situ: immunofluorescent and immunoenzymatic techniques
Isolation, characterisation and cultivation of
human monocytes and macrophages