Cilia, Part A

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cilia and includes chapters on such topics as electron microscopy of IFT in cilia and flagella, radial spoke isolation and assays, and biomechanical measurements of kinocilium.

Continues the legacy of this premier serial with quality chapters authored by leaders in the fieldCovers ciliaContains chapters on such topics as electron microscopy of IFT in cilia and flagella, radial spoke isolation and assays, and biomechanical measurements of kinocilium

1. Electron microscopy of IFT in cilia and flagella
   
2. Biochemical analysis of ciliary transport
3. Visualization of IFT in Chlamydomonas flagella
4. Visualization of kidney cilia-mediated calcium signaling
5. Methods for analyzing nodal cilia and flow
6. Methods for building synthetic cilia-like motor-microtubule arrays
7. Isolation of IFT particle proteins from Chlamydomonas
8. Radial Spoke isolation and assays
9. Biomechanical measurements of kinocilium
10. Methods for analysis of Calcium/calmodulin signaling in flagella
11. Ubiquitination of Flagellar Proteins
12. Visualizing IFT in C. elegans cilia
13. Proteomic analysis of mammalian primary cilia
14. TSC and TOR signaling in cilia
15. Bioinformatic approaches to cilia
16. Methods for testing cilia function in cell migration
17. Signaling and waveform in Drosophila sperm flagella
18. Analysis of import into primary cilia
19. Patch-Clamp Recording from Mouse and Human Spermatozoa
20. Optical trap measurements of flagellar membrane motility