Molecular Biology: A Project Approach

This course manual instructs students in recombinant DNA techniques and other essential molecular biology techniques in the context of projects. The project approach inspires and captivates students; it involves them in the scientific experience, providing continuity to laboratory bench time and an understanding of the principles underlying the techniques presented.

Molecular Biology is a must for any department, operating under budgetary constraints that offers or plans to offer a course in molecular cloning.

Includes a glossary of over 200 terms important for understanding molecular biologyUses an inexpensive source of eukaryotic cells - great for schools on a budgetIncludes Methods Locator that provides instant access to the latest methodsContain clearly written, easy-to-follow, student-tested instructions: Sterile techniquesPhage titrationGel electrophoresis of DNARestriction enzyme digestionPlasmid isolationTransformation of E. ColiRecombinant DNA cloningNick translation labelingNonradioactive primer labellingNonradioactive DNA detectionSouthern blottingColony hybridizationPurification of plant DNARNA purificationNorthern blottingPurification of poly A+ RNAPolymerase chain reaction (PCR)

Transposon Mutagenesis of Escherichia coli
Recombinant DNA and Cloning
Southern Blot Analysis
Plant Genomic Southern Blotting with Probes for Low- and High-Copy-Number Genes
RNA Purification and Northern Blot Analysis
Polymerase Chain Reaction