Caenorhabditis elegans: Cell Biology and Physiology

The second part of an updated edition of the classic Methods in Cell Biology, Volume 48, this book emphasizes diverse methods and technologies needed to investigate C. elegans, both as an integrated organism and as a model system for research inquiries in cell, developmental, and molecular biology, as well as in genetics and pharmacology. By directing its audience to tried-and-true and cutting-edge recipes for research, this comprehensive collection is intended to guide investigators of C. elegans for years to come.

Diverse, up-to-date techniques covered will be useful to the broadening community of C. elegans researchers for years to comeChapters written by leaders in the fieldTried and true methods deliver busy researchers a one-stop compendium of essential protocols

1.Imaging

High-resolution Imaging of Cellular Processes in C. elegans Immunofluorescence Microscopy Fluorescent Protein Methods: Strategies and Applications Modern Electron Microscopy Methods for C. elegans

2. Cell Biology

Culture and Manipulation of Embryonic Cells Laser Microsurgery in Caenorhabditis elegans Analysis of Cell polarity Analysis of Organelles C. elegans Cell Cycle Analysis

3. Physiology

Methods for Studying Programmed Cell Death in C. elegans Methods for Studying the DNA Damage Response in the Caenorhabditis elegans Germline Analysis of Aging in Caenorhabditis elegans Analyses of C. elegans Fat Metabolic Pathways Electrophysiological Methods for C. elegans Neurobiology