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Imaging and Spectroscopic Analysis of Living Cells: Live Cell Imaging of Cellular Elements and Functions

ISBN 9780123884480

Autor: Conn, P. Michael

Wydawca: Elsevier

Dostępność: 3-6 tygodni

Cena: 764,40 zł

Przed złożeniem zamówienia prosimy o kontakt mailowy celem potwierdzenia ceny.

ISBN13:	9780123884480
ISBN10:	0123884489
Autor:	Conn, P. Michael
Oprawa:	Hardback
Rok Wydania:	2012-03-15
Tematy:	MJ

This volume of Methods in Enzymology is the second of three parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike.

Expert authors who are leaders in the fieldExtensively referenced and useful figures and tablesProvides hints and tricks to facilitate reproduction of methods

Part A: Organelles and Cellular Structure

Monitoring cytoskeletal dynamics in living neurons using fluorescence photoactivation
Use of DNA-specific anthraquinone dyes to directly reveal cytoplasmic and nuclear boundaries in live and fixed cells
Methods to measure actin treadmilling rate in dendritic spines
Dynamics of Clathrin coat formationImaging mitotic spindle dynamics
Dynamics of microdomains
Imaging chromosome dynamics in meiosis
Analyzing lysosomes in live cells
Imaging of mobile long-lived nanoplatforms in the live cell plasma membrane
Visualizing ion channel dynamics at the plasma membrane
Live imaging studies of the cytoskeleton
Fluorescence single-molecule imaging of actin turnover and regulatory mechanisms
Analysis of cell migration and cell-to-cell interaction by videomicroscopy

Part B: Molecules and Ions

Real-time quantitative and qualitative assay for Glut-4 translocation
Live-cell imaging with signalling complexes
Dynamic imaging of oligomerization in living cells
Time-resolved liminescence resonance energy transfer imaging of protein—protein interactions in living cells
Imaging steriod receptors in live cells
Investigating second messenger signaling in vivo
Probes for intracellular RNA imaging in live cells
Imaging the glycome in live cells
Intracellular magnesium detection
Imaging mobile zinc
Imaging ion flux and ion homeostasis in live cells