Fluorescence-Based Biosensors: From Concepts to Applications

One of the major challenges of modern biology and medicine consists in finding means to visualize biomolecules in their natural environment with the greatest level of accuracy, so as to gain insight into their properties and behaviour in a physiological and pathological setting. This has been achieved thanks to the design of novel imaging agents, in particular to fluorescent biosensors.

Fluorescence Biosensors comprise a large set of tools which are useful for fundamental purposes as well as for applications in biomedicine, drug discovery and biotechnology. These tools have been designed and engineered thanks to the combined efforts of chemists and biologists over the last decade, and developed hand in hand together with imaging technologies.

This volume will convey the many exciting developments the field of fluorescent biosensors and reporters has witnessed over the recent years, from concepts to applications, including chapters on the chemistry of fluorescent probes, on technologies for monitoring protein/protein interactions and technologies for imaging biosensors in cultured cells and in vivo. Other chapters are devoted to specific examples of genetically-encoded reporters, or to protein and peptide biosensors, together with examples illustrating their application to cellular and in vivo imaging, biomedical applications, drug discovery and high throughput screening.

• Fluorescence technologies for monitoring interactions between biological macromolecules

• Design and Synthesis of Fluorogenic Molecules

• Fluorescent environment-sensitive dyes as reporters of biomolecular interactions

• Rational Design of Fluorophores for In vivo Imaging

• Fluorescent sensors of protein kinases

• Time-resolved FRET-based screening methods for GPCR

• Fluorescent proteins as sensors for tumor growth and metastasis

• Fluorescent macromolecular sensors of enzymatic activity for in vivo imaging

• Fluorescent protein-based biosensors and their clinical application

• Microscopy for measurement of fluorescence-based reporters in living cells; analysis and methodologies for kinase activity assessment