Biology of Serpins

Serpins are a group of proteins with similar structures that were first identified as a set of proteins able to inhibit proteases. The acronym serpin was originally coined because many serpins inhibit chymotrypsin-like serine proteases. This volume of Methods in Ezymology is split into 2 parts and comprehensively covers the subject.

1. Analysis of Serpin Secretion, Misfolding and Surveilance in the Endoplasmic Reticulum

Shujuan Pan, Michael J. Iannotti and Richard N. Sifers

2. Serpin-Enzyme Receptors: Ldl Receptor Related Protein 1

Dudley K. Strickland, Selen Catania Muratoglu and Toni M. Antalis

3. The Role of Autophagy in Alpha-1-Antitrypsin Deficiency

Tunda Hidvegi, Amitava Mukherjee, Michael Ewing, Carolyn Kemp and David H. Perlmutter

4. Serpins and the Complement System

László Beinrohr, Thomas A. Murray-Rust, Leanne Dyksterhuis, Péter Závodszky, Péter Gál, Robert N. Pike and Lakshmi C. Wijeyewickrema

5. Use of Mouse Models to Study Plasminogen Activator Inhibitor-1

Paul J. Declerck, Ann Gils and Bart De Taeye

6. Plasminogen Activator Inhibitor Type 2: Still An Enigmatic Serpin But A Model For Gene Regulation

Robert L. Medcalf

7. The Serpinb1 Knock-Out Mouse: A Model For Studying Neutrophil Protease Regulation in Homeostasis and Inflammation

Charaf Benarafa

8. Investigating Maspin in Breast Cancer Progression Using Mouse Models

Michael P. Endsley and Ming Zhang

9. Hsp47as A Collagen-Specific Molecular Chaperone

Yoshihito Ishida and Kazuhiro Nagata

10. Assays for the Antiangiogenic and Neurotrophic Serpin Pigment Epithelium-Derived Factor

S. Patricia Becerra, Susan E. Crawford and Preeti Subramanian

11. The Drosophila Serpins: Multiple Functions in Immunity and Morphogenesis

Jean Marc Reichhart, David Gubb and Vincent Leclerc

12. Modelling Serpin Conformational Diseases in Drosophila Melanogaster

Thomas R. Jahn, Elke Malzer, John Roote, Anastasia Vishnivetskaya, Sara Imarisio, Maria Giannakou, Karin Panser, Stefan Marciniak, Damian C. Crowther

13. Using Caenorhabditis Elegans to Study Serpinopathies

Olivia S. Long, Sager J. Gosai, Joon Hyeok Kwak, Dale E. King, David H. Perlmutter, Gary A. Silverman and Stephen C. Pak

14. Using c. Elegans to Identify the Protease Targets of Serpins In Vivo.

Sangeeta R. Bhatia, Mark T. Miedel, Cavita K. Chotoo, Nathan J. Graf, Brian L. Hood, Thomas P. Conrads, Gary A. Silverman and Cliff J. Luke

15. Viral Serpin Therapeutics: From Concept to Clinic

Hao Chen, MD, PhD, Donghang Zheng, MD, PhD, Jennifer Davids, BA, Mee Yong Bartee, PhD, Erbin Dai, MD, Liying Liu, MD, Lyubomir Petrov, MD, PhD, Colin Macaulay, PhD, Robert Thoburn, MD, Eric Sobel, MD, PhD, Richard Moyer, PhD, Grant McFadden, PhD, Alexandra Lucas, MD, FRCP(C)

16. Human Scca Serpins Inhibit Staphylococcal Cysteine Proteases by Forming Classic "Serpin-Like" Covalent Complexes

Tomasz Kantyka and Jan Potempa

17. Plants and the Study of Serpin Biology

Thomas H. Roberts, Joon-Woo Ahn, Nardy Lampl and Robert Fluhr