Human Stem Cell Manual: A Laboratory Guide

This manual is a comprehensive compilation of "methods that work" for deriving, characterizing, and differentiating hPSCs, written by the researchers who developed and tested the methods and use them every day in their laboratories. The manual is much more than a collection of recipes; it is intended to spark the interest of scientists in areas of stem cell biology that they may not have considered to be important to their work. The second edition of the Human Stem Cell Manual is an extraordinary laboratory guide for both experienced stem cell researchers and those just beginning to use stem cells in their work.

Offers a comprehensive guide for medical and biology researchers who want to use stem cells for basic research, disease modeling, drug development, and cell therapy applicationsProvides a cohesive global view of the current state of stem cell research, with chapters written by pioneering stem cell researchers in Asia, Europe, and North AmericaIncludes new chapters devoted to recently developed methods, such as iPSC technology, written by the scientists who made these breakthroughs

Foreword by James Battey
Foreword by Jeannie Fontana
Preface
Part I: Basic methods: Maintenance of stem cell cultures
1.Human Embryonic Stem Cell Culture 
2.Feeder layers and feeder-free culture
3.Mouse Embryonic Fibroblast Feeder Cells 
4.Cryopreservation of hESC
Part II: Basic methods: Characterization of stem cells
5.Classical Cytogenetics: Karyotyping
6.Spectral Karyotyping and Fluorescent in situ Hybridization 
7.Using SNP Genotyping to Identify Cell Lines, Determine Genomic Abnormalities and Monitor DNA Methylation Patterns
8.FACS/Cell sorting
9.Fluorescence Immunocytochemical Analysis of Stem Cells 
10.Characterization of Stem Cells Using RT-PCR
11.Gene Expression Profiling of Stem Cells by Microarray
12.Teratomas produced from Human Embryonic Stem Cells Xenografted into Immunodeficient Mice 
13.Generation of HESC-Derived Teratomas 
Part III: Differentiation of stem cells
14.“Embryoid Body” Formation and Neuroepithelia…