Cryo-EM, Part C: Analyses, Interpretation, and Case Studies

This volume, along with Part A and Part B, is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied.

* Includes time-tested core methods and new innovations applicable to any researcher * Methods included are useful to both established researchers and newcomers to the field * Relevant background and reference information given for procedures can be used as a guide

1. Analyses of Subnanometer Resolution Cryo-EM Density Maps

Matthew L. Baker, Mariah R. Baker Corey F. Hryc Frank DiMaio

2. Methods for Segmentation and interpretation of cryo-EM data

Niels Volkmann

3. Integration of Cryo-EM with atomic and protein: protein interaction data

Friedrich Forster

4. Unified Data Resource for CryoEM

Catherine L. Lawson

5. Electron crystallography and aquaporins

Thomas Walz, Andreas D. Schenk, Richard K. Hite, Andreas Engel, Yoshinori Fujiyoshi

6. Cryo-Electron Microscopy Applications in the Study of Tubulin Structure, Microtubule Architecture, Dynamics and Assemblies, and Interaction of Microtubules with Motors

Kenneth H. Downin and Eva Nogales

7. Helical crystallization of two example membrane proteins: MsbA and the Ca2+-ATPase

Howard S. Young, John Paul Glaves, Lauren Fisher, Andrew Ward

8. Multi-particle cryo-EM of ribosomes

Christian M.T. Spahn, Justus Loerke, Jan Giesebrecht

9. Single-particle electron microscopy of animal fatty acid synthase: Describing macromolecular rearrangements that enable catalysis

Francisco Asturias, Edward J. Brignole

10. Tomography of actin cytoskeletal networks

Dorit Hanein

11. Visual Proteomics

Friedrich Förster, Bong-Gyoon Han, and Martin Beck

12. Cryo-Electron Tomography of Eukaryotic Cells

Ohad Medalia, Asaf Mader , Nadav Elad

13. 3d Visualization Of Hiv Virions By Cryo Electron Tomography

Jun Liu, Elizabeth R. Wright, Hanspeter Winkler

14. Automation in Single-Particle Electron Microscopy: Connecting the Pieces

Bridget Carragher, Dmitry Lyumkis, Arne Moeller, Anchi Cheng, Amber Herold, Eric Hou, Christopher Irving, Erica L. Jacovetty, Pick-Wei Lau, Anke Mulder, James Pulokas, Joel D. Quispe, Neil R. Voss, Clinton S. Potter