The Zebrafish: Cellular and Developmental Biology, Part A

This volume of Methods in Cell Biology, the first of 3 parts on the subject of zebrafish, provides a comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 2004. This first volume provides state-of-the-art descriptions of novel cellular imaging technologies and methods for culture of zebrafish stem cells, summarizes protocols for analyzingthe development of major organ systems including the central nervous system (CNS), and introduces the use of the zebrafish as a model system for human diseases.

Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfallsSummarizes the Zebrafish Genome Project

Part I: Cell Biology

1. Cytoskeletal Analysis of Early Zebrafish Embryos

Martin Wühr, William H. Detrich III, and Timothy J. Mitchison

2. Development of Zebrafish Progenitor Cultures

David Traver

3. Axon Guidance in the Zebrafish

Chi-Bin Chien

4. Imaging Blood Vessels in the Zebrafish

Weinstein

5. Lipid Metabolism in Zebrafish

Steven Farber

6. Analysis of the Cell Cycle in Zebrafish

James Amatruda

7. Medaka Haploid Cells

Hong Wunhan

8. Ciliary Transport

Jarema Malicki and Zhoxia Sun

Part II: Developmental and Neural Biology

9. Light Sheet Microscopy

Ernst Steltzer and Jochen Wittbrodt

10. Multiscale Dynamics in Zebrafish Embryogenesis

Nadine Peyriéras

11. The caspar Mutant in Organogenesis

Len Zon

12. Neurogenesis

Prisca Chapouton and Leanne Godinho

13. Development of the Peripheral Sympathetic Nervous SystemRodney Stewart, A. Thomas Look, John Kanki, and Paul Henion
14. Enteric Nervous System

Judith Eisen, Iain Shepherd

15. Electrophysiological Methods for the Lateral Line

Theresa Nicholson

16. Computer-Aided Screening for Zebrafish Embryonic Motility Mutants

Charles Lessman

17. Photoreceptor Structure and Development: Analyses Using GFP Transgenes

Brian Perkins, James Fadool, and John Dowling

18. Approaches to Study Neurogenesis in the Zebrafish Retina

Andrei Avanesov and Jarema Malicki

19. Morphogenesis of the Jaw: Development Beyond the Embryo

R. Craig Albertson and Pamela Yelick

20. Cardiac Development

Deb Yelon and Grant Miura

21. Chemical Approaches to Angiogenesis

Joann Chan

22. Vascular Occlusion and Thrombosis in Zebrafish

Pudur Jagadeeswaran and Bijoy Thattaliyath

23. Zebrafish Kidney Development

Iain Drummond and Alan Davidson

24. Pancreas Development in Zebrafish

Dirk Meyer and Vicki Prince

25. Gut

Didier Stainier and Wolfram Gosling

26. Sleep

Alex Schier

27. Associative Learning in Zebrafish

Lina Al-Imari and Robert Gerlai