Techniques in Confocal Microscopy

As part of the Reliable Lab Solutions series, Techniques in Confocal Microscopy brings together chapters from volumes 302, 307 and 356 of Methods in Enzymology. It documents many diverse uses for confocal microscopy in disciplines that broadly span biology.

Documents many diverse uses for confocal microscopy in disciplines that broadly span biology The methods presented include shortcuts and conveniences not included in the initial publications Techniques are described in a context that allows comparisons to other related methodologiesMethodologies are laid out in a manner that stresses their general applicability and reports their potential limitations

Part I. Practical Considerations and Equipment

1. Theoretical Basis of Confocal Microscopy Robert H. Webb

2. Practical Considerations in Acquiring Biological Signals from Confocal Microscope Hao He, Rose Chik Ying Ong, Kam Tai Chan, Ho Pui Ho, Siu Kai Kong

3. Equipment for Mass Storage and Processing of Data Guy Cox

4. Antifading Agents for Confocal Fluorescence Microscopy Miguel Berrios, Kimberly A. Conlon, and David E. Colflesh

5. Mounting Techniques for Confocal Microscopy Manabu Kagayama and Yasuyuki Sasano

6. Preparation of Whole Mounts and Thick Sections for Confocal Microscopy Hisashi Hashimoto, Hiroshi Ishikawa, and Moriaki Kusakabe   7. Use of Confocal Microscopy to Investigate Cell Structure and Function Ghassan Bkaily, Danielle Jacques

8. Combining Laser Scanning Confocal Microscopy and Electron Microscopy Xuejun Sun

Part II. Functional Approaches

9. Volume Measurements in Confocal Microscopy Carlos B. Mantilla, Y.S. Prakash and Gary C. Sieck

10. Quantitation of Phagocytosis by Confocal Microscopy George F. Babcock and Chad T Robinson MS

11. Receptor-Ligand Internalization Guido Orlandini, Nicoletta Ronda, Rita Gatti, Gian Carlo Gazzola, and Alberico Borghetti

12. Quantitative Imaging of Metabolism by Two-Photon Excitation Microscopy David W. Piston and Susan M. Knobel

13. Trafficking of the Androgen Receptor Virginie Georget, Béatrice Terouanne, Jean-Claude Nicolas, and Charles Sultan

14. Localization of Proteases and Peptide Receptors by Confocal Microscopy Kanti D. Bhoola, Celia J. Snyman and Carlos D. Figueroa

15. Multiphoton Excitation Microscopy, Confocal Microscopy, and Spectroscopy of Living Cells and Tissues: Functional Metabolic Imaging of Human Skin in Vivo Barry R. Masters, Peter T. C. So, Ki Hean Kim, Christof Buehler, and Enrico Gratton

16. Video-Rate, Scannng Slit Confocal Microscopy of Living Human Cornea in Vivo: Three-Dimensional Confocal Microscopy of the Eye Barry R. Masters and Matthias Böhnke

17. In Vivo Imaging of Mammalian Central Nervous System Neurons with the In Vivo Confocal Neuroimaging (ICON) Method Sylvia Prilloff, Petra Henrich-Noack, Ralf Engelmann, Bernhard A. Sabel

18. Identification of Viral Infection by Confocal Microscopy David N. Howell and Sara E. Miller

19. Membrane Trafficking Sabine Kupzig, San San Lee, and George Banting

III. Green Fluorescent Protein

20. Monitoring of Protein Secretion with Green Fluorescent Protein Christoph Kaether and Hans-Hermann Gerdes

21. Comparison of Enhanced Green Fluorescent Protein and Its Destabilized Form as Transcription Reporters Xiaoning Zhao, Tommy Duong, Chiao-Chian Huang, Steven R. Kain, and Xianqiang Li

22. Measuring Protein Degradation with Green Fluorescent Protein Stephen R. Cronin and Randolph Y. Hampton

23. Studying Nuclear Receptors with Green Fluorescent Protein Fusions Gordon L. Hager

24. Signaling, Desensitization, and Trafficking of G Protein-Coupled Receptors Revealed by Green Fluorescent Protein Conjugates Larry S. Barak, Jie Zhang, Stephen S. G. Ferguson, Stephane A. Laporte, and Marc G. Caron

25. Fluorescent Proteins in Single- and Multicolor Flow Cytometry Lonnie Lybarger and Robert Chervenak

26. Jellyfish Green Fluorescent Protein: A Tool for Studying Ion Channels and Second-Messenger Signaling in Neurons L. A. C. Blair, K. K.Bence, and J. Marshall

27. Expression of Green Fluorescent Protein and Inositol 1,4,5-Triphosphate Receptor in Xenopus laevis Oocytes Atshushi Miyawaki, Julie M. Matheson, Lee G. Sayers, Akira Muto, Takayuki Michikawa, Teiichi Furuichi, and Katsuhiko Mikoshiba

28. Confocal Imaging of Ca2+, pH, Electrical Potential, and Membrane Permeability in Single Living Cells John J. Lemasters, Donna R. Trollinger, Ting Qian, Wayne E. Cascio, and Hisayuki Ohata

Part IV.  Laser Capture Microdissection

29. Laser Capture Microdissection and Its Applications in Genomics and Proteomics James L. Wittliff

30. Going in Vivo with Laser Microdissection Anette Mayer, Monika Stich, Dieter Brocksch, Karin Schütze, and Georgia Lahr

31. Flourescence in Situ Hybridization of LCM-Isolated Nuclei from Paraffin Sections Douglas J. Demetrick, Sabita K. Murthy, and Lisa M. DiFrancesco