Essential Ion Channel Methods

The rapid growth of interest and research activity in ion channels is indicative of their fundamental importance in the maintenance of the living state. This volume was prepared with a view toward providing a sampling of the range of molecular and physical methods that are significant for the study of ion channels.

As part of the Reliable Lab Solutions series, Essential Ion Channel Methods brings together chapters from volumes 293 and 294 of Methods in Enzymology. The chapters have been selected by the editor and updated, when possible, by their original authors to include new research and references. The result is a set of chapters which make use of graphics, comparisons to other methods, and provide tricks and approaches that make it possible to adapt methods to other systems. Methods are presented in a fashion that allows their replication by individuals new to the field, yet providing valuable information for seasoned investigators.

Highlights top downloaded and cited chapters, authored by pioneers in the field and enhanced with graphics and easy to follow methodsLoaded with detailed protocols developed and used by leaders in the fieldRefines, organizes and updates popular methods from one of our top selling series, Methods in Enzymology

PART I - Assembly 1. Assembly of Ion Channels ZuFang Sheng and Carol Deutsch

PART II - Genetics 2. Identification of Ion Channel-Associated Proteins Using the Yeast Two-Hybrid System Martin Niethammer and Morgan Sheng

PART III - Electrophysiology 3. Patch Clamp Studies of the Cystic Fibrosis Transmembrane Conductance Regulator Chloride Channel John W. Hanrahan, Zie Kone , Ceri J. Mathews,Jiexin Luo, Yanlin Jia  and Paul Lindsell

4. Tight-Seal Whole-cell Patch Clamping of C. elegans Neurons S.R. Lockery and M.B. Goodman

5. Gating Currents Enrico Stefani and Francisco Bezanilla

6. Determining Ion Channel Permeation Properties Ted Begenisich

PART IV - Expression Systems 7. Expression of Ligand-Gated Ion Channels Using Using Semliki Forest Virus and Baculovirus Kathryn Radford and Gary Buell

8. Recombinant Adenovirus-Mediated Expression in Nervous System of Genes Coding for Ion Channels and Other Molecules Involved in Synaptic Function Markus U. Ehrengruber, Markus Lanzrein, Youfeng Xu, Mark C. Jasek, David B. Kantor, Erin M. Schuman, Henry A. Lester, and Norman Davidson

9. Transient Expression of Heteromeric Ion Channels Alison L. Eertmoed, Yolanda F. Vallejo, and William N. Green

PART V - Model Simulations 10. Computer Simulations and Modeling of Ion Channels Michael E. Green

PART VI.  Physical 11. Fluorescence Techniques for Measuring Ion Channel Activity Gönül Velicelebi, Kenneth A. Stauderman, Mark A. Varney, Michael Akong, Stephen D. Hess, and Edwin C. Johnson

12. Ligand Binding Methods for Analysis of Ion Channel Structure and Function Steen E. Pedersen, Monica M. Lurtz, and Rao V. L. Papineni

13. Three-dimensional structure of membrane proteins determined by two-dimensional crystallization, electron cryomicroscopy, and image analysis Mark Yeager, Vinzenz M. Unger, Alok K. Mitra

14. Voltage Clamp Biosensors for Capillary Electrophoresis Owe Orwar, Kent Jardemark, Cecilia Farre, Ingemar Jacobson, Alexander Moscho, Jason B. Shear, Harvey A. Fishman, Sheri J. Lillard, Richard N. Zare

15. Ion Channels As Tools to Monitor Lipid Bilayer-Membrane Protein Interactions: Gramicidin Channels As Molecular Force O. S. Andersen, C. Nielsen, A. M. Maer, J. A. Lundbæk, M. Goulian, and R. E. Koeppe I

PART VII. Purification and Reconstitution 16. Purification and Reconstitution of the Epithelial Chloride Channel Cystic Fibrosis Transmembrane Conductance Regulator Mohabir Ramjeesingh, Elizabeth Garani, Kevin Galley, Canhui Li, Yanchun Wang, Christine E. Bear

17. Reconstitution of Native and Cloned Channels into Planar Bilayers I. Favre, Y.-M. Sun, and E. Moczydlowski

PART VIII. Second Messengers and Biochemical Approaches 18. Protein Phosphorylation of Ligand-Gated Ion Channels Andrew l. Mammen, Sunjeev Kamboj, and Richard L. Huganir

19. Analysis of Ion Channel Associated Proteins Michael Wyszynski and Morgan Sheng

20. Secondary Messenger Regulation of Ion Channels/Plant Patch Clamping Sarah M. Assmann and Lisa Romano

PART IX. Special Channels 21. ATP-Sensitive Potassium Channels Joseph Bryan, M. Schwanstecher, C. Schwanstecher, F. Chudziak, U. Panten, J.P. Clement IV, G. Gonzalez, and L. Aguilar-Bryan

22. Simplified Fast Pressure-Clamp Technique for Studying Mechanically-Gated Channels Don W. McBride, Jr. and Owen P. Hamill

23. Purification and Heterologous Expression of Inhibitory Glycine Receptors Bodo Laube and Heinrich Betz

24. Functional Analyses of Aquaporin Water Channel Proteins Peter Agre, John C. Mathai, Barbara L. Smith, and Gregory M. Preston

PART X. Toxins and Other Membrane Active Compounds 25. Conus Peptides as Probes for Ion Channels J. Michael McIntosh, Baldomero M. Olivera, and Lourdes J. Cruz

26. Scorpion Toxins as Tools for Studying Potassium Channels Maria Garcia, Markus Hanner, Hans-Günther Knaus, Robert Slaughter, Gregory J. Kaczorowski

27. The Use of Planar Lipid Bilayer Membranes for Rapid Screening of Membrane Active Compounds Bruce L. Kagan, Tajb A. Mirzabekov, Antoly Y. Silberstein

28. Antibodies to Ion Channels Angela Vincent, Ian Hart, Ashwin Pinto, and F. Anne Stephenson