Microtubules: in vivo

Microtubules: in vivo includes chapters by experts around the world on many aspects of microtubule imaging in living and fixed cells; assays to study microtubule function in a wide array of model organisms and cultured cells; high resolution approaches to study of the cytoskeleton. The authors share their years of experience, outlining potential pitfalls and critical factors to consider in experimental design, experimental implementation and data interpretation.

Includes chapters by experts around the world on many aspects of microtubule imaging in living and fixed cells; assays to study microtubule function in a wide array of model organisms and cultured cells; high resolution approaches to study of the cytoskeletonThe authors share their years of experience, outlining potential pitfalls and critical factors to consider in experimental design, experimental implementation and data interpretation

1. Microtubule dynamics at the cell cortex probed by TIRF microscopy

Ilya Grigoriev and Anna Akhmanova

2. Microscopy methods for the study of centriole biogenesis and function in Drosophila

Ana Rodrigues Martins, Pedro Machado, Giuliano Callaini and Monica Bettencourt-Dias

3. Quality control in single molecule studies of kinesins and microtubule-associated proteins

Gary J. Brouhard

4. Imaging cilia in zebrafish

Kimberly M. Jaffe, Stephan Y. Thiberge, Margaret E. Bisher and Rebecca D. Burdine

5. Automatic Identification of Microtubules in Cellular Electron Tomography

Daniyar Nurgaliev, Timur Gatanov and Daniel J. Needleman

6. Imaging and analysis of the microtubule cytoskeleton in Giardia

Scott C. Dawson and Susan A. House

7. Kinetochore-microtubule dynamics and attachment stability

Jennifer G. DeLuca

8. Assessment of mitotic spindle phenotypes in Drosophila S2 cells

Gohta Goshima

9. Live cell imaging techniques for analyses of microtubules in Dictyostelium

Matthias Samereier, Irene Meyer, Michael P. Koonce and Ralph Gräf

10. Microtubule dynamics in dendritic spines

Lukas C. Kapitein , Kah Wai Yau and Casper C. Hoogenraad

11. Analysis of Microtubules in Budding Yeast

Alexander Rauch, Elena Nazarova and Jackie Vogel

12. Determination of Microtubule Dynamic Instability in Living Cells

Kathy Kamath, Emin Oroudjev, and Mary Ann Jordan

13. Modeling microtubule-mediated forces and centrosome positioning in Caenorhabditis elegans embryos

Akatsuki Kimura1 and Shuichi Onami

14. Cryo electron tomography of cellular microtubules

Roman I. Koning

15. Microtubule dynamics in plant cells

Henrik Buschmann, Adrian Sambade, Edouard Pesquet, Grant Calder and Clive W. Lloyd

16. Drosophila S2 cells as a model system to investigate mitotic spindle dynamics, architecture and function

Sara Moutinho-Pereira, Irina Matos and Helder Maiato

17. Protein micropatterns: a direct printing protocol using deep UVs

Ammar Azioune, Nicolas Carpi, Manuel Théry and Matthieu Piel

18. Optical trapping and laser ablation of microtubules in fission yeast

Nicola Maghelli and Iva M. Tolić-Nørrelykke

19. Melanophores for Microtubule dynamics and motility assays

Kazuho Ikeda, Irina Semenova, Olga Zhapparova, and Vladimir Rodionov

20. New and old reagents for fluorescent-protein tagging of microtubules in fission yeast: experimental and critical evaluation

Hilary A. Snaith, Andreas Anders, Itaru Samejima and Kenneth E. Sawin

21. Imaging of mitotic spindle dynamics in C. elegans embryos

Mika Toya, Yumi Iida and Asako Sugimoto

22. A fast microfluidic temperature control device for studying microtubule dynamics in fission yeast

Guilhem Velve-Casquillas, Judite Costa, Frédérique Carlier-Grynkorn , Adeline Mayeux and Phong T. Tran

23. Microtubule-dependent spatial organization of mitochondria in fission yeast

Maitreyi Das, Stephan Chiron and Fulvia Verde

24. Photoactivatable GFP-Tubulin

U. Serdar Tulu*, Nick P. Ferenz

25. The Use of Fluorescence Redistribution After Photobleaching for Analysis of Cellular Microtubule Dynamics

Claire E. Walczak, Rania S. Rizk and Sidney L. Shaw

26. Analysis of microtubule polymerization dynamics in live cells

Sarah Gierke, Praveen Kumar, and Torsten Wittmann