Nuclear Mechanics and Genome Regulation

In recent years new discoveries have made this an exciting and important field of research. This exhaustive volume presents comprehensive chapters and detailed background information for researchers working with in the field of nuclear mechanics and genome regulation.

Both classic and state-of-the-art methods readily adaptable and designed to last the test of timeRelevant to clinicians and scientists working in a wide range of fields

Section-A: Cell Nucleus: Organization and MechanoBiology

1. Fluorescence Fluctuation Microscopy to reveal 3D-Architecture and Function in the Cell Nucleus

Thorsten Lenser^1,2, Klaus Weisshart²_, Tobias Ulbricht³, Karolin Klement³, and Peter Hemmerich

2. Studying histone modifications and their genomic functions by employing chromatin immunoprecipitation and immunoblotting

Ranveer S. Jayani, Praveena L. Ramanujam and Sanjeev Galande

3. Dynamic organization of chromatin assembly & transcription factories in living cells

Bidisha Sinha, Dipanjan Bhattacharya, Deepak Kumar Sinha, Shefali Talwar,

Shovamayee Maharana, Soumya Gupta and G. V.Shivashankar

4. Manipulation & Isolation of Single Cells & Nuclei

Swee Jin Tan, Qingsen Li and Chwee Teck Lim

5. Beyond lamins: other structural components of the nucleoskeleton

Zhixia Zhong , Katherine L. Wilson and Kris Noel Dahl

6. Altered mechanical properties of the nucleus in disease

Maria Lucia Lombardi and Jan Lammerding

7. Theoretical concepts and models of cellular mechanosensing

Rumi De, Assaf Zemel and Samuel A. Safran

Section-B: Impact of Nuclear Mechanics on Function

8. Mechanical Induction of Gene Expression in Connective Tissue Cells

Mathew Chan, Boris Hinz, Christopher A. McCulloch

9. Physical plasticity of the Nucleus and its manipulation

Irena Ivanovska, Joe Swift, Takamasa Harada, J. David Pajerowski and Dennis E. Discher

10. Prestressed Nuclear Organization in Living Cells

Aprotim Mazumder, Roopa T. , Abhishek Kumar, K.Venkatesan Iyer,

Nisha M Ramdas and G. V.Shivashankar

11. Nanotopography/mechanical induction of stem-cell differentiation

Evelyn Yim

12. Mechanical Induction in Embryonic Development and Tumour Growth: Integrative Cues through Molecular to Multi-cellular Interplay, and Evolutionary Perspectives

Maria-Elena Fernandez-Sanchez, Fanny Serman, Padra Ahmadi and Emmanuel Farge

13. Informatics based analysis of mechanosignaling in the laminopathies

Frank P.L. Lai, Radfidah A. Mutalif, Siew Cheng Phua, and Colin L. Stewart

14. Autosomal Dominant Leukodystrophy caused by lamin B1 duplications: A clinical and molecular case study of altered nuclear function and disease

Quasar Saleem Padiath and Ying-Hui Fu