Cilia: Motors and Regulation

Along with its companion volume on axonemal dynein-mediated motility, this book provides researchers with a comprehensive and up-to-date source of methods for the analysis cilia and flagella, focusing primarily on approaches that have been devised or significantly extended since the last volume of Methods in Cell Biology on this topic (volume 47, 1995). Edited by Stephen M. King and Gregory J. Pazour, the newest installment of this highly acclaimed serial will serve as an essential addition to the study of cilia and flagella.

* Covers protocols for cilia and flagella across systems and species * Both classic and state-of-the-art methods readily adaptable across model systems, and designed to last the test of time * Relevant to clinicians interested in respiratory disease, male infertility, and other syndromes who need to learn biochemical, molecular, and genetic approaches to studying cilia, flagella, and related structures

MCB, 92

1. Bioinformatic Approaches to Dynein Heavy Chain Classification

Toshiki Yagi

2. Identification and Characterization of Dynein Genes in Tetrahymena

David E. Wilkes, Nicole Bennardo, Clarence W.C. Chan, Yu-Loung Chang,

Elizabeth O. Corpuz, Jennifer DuMond, Jordan A. Eboreime, Julianna Erickson,

Jonathan Hetzel, Erin E. Heyer, Mark J. Hubenschmidt, Ekaterina Kniazeva,

Hallie Kuhn, Michelle Lum, Andrea Sand, Alicia Schep, Oksana Sergeeva,

Natt Supab, Caroline R. Townsend, Liesl Van Ryswyk, Hadley E. Watson,

Alice E. Wiedeman, Vidyalakshmi Rajagopalan, and David J. Asai

3. Purification of Axonemal Dyneins and Dynein-Associated Components

from Chlamydomonas

Stephen M. King

4. Purification of Dyneins from Sperm Flagella

Kazuo Inaba and Katsutoshi Mizuno

5. Protein Engineering Approaches to Study the Dynein Mechanism

using a Dictyostelium Expression System

Takahide Kon, Tomohiro Shima, and Kazuo Sutoh

6. Biophysical Measurements on Axonemal Dyneins

Hiroaki Kojima, Shiori Toba, Hitoshi Sakakibara, and Kazuhiro Oiwa

7. Protein Electroporation into Chlamydomonas for Mutant Rescue

Masahito Hayashi and Ritsu Kamiya

8. Analysis of the Role of Nucleotides in Axonemal Dynein Function

Chikako Shingyoji

9. The Regulation of Dynein-Driven Microtubule Sliding in Chlamydomonas

Flagella by Axonemal Kinases and Phosphatases

Candice A. Elam, Winfield S. Sale, and Maureen Wirschell

10. Analysis of Redox-Sensitive Dynein Components

Ken-ichi Wakabayashi

11. Calcium Regulation of Ciliary Motility: Analysis of Axonemal

Calcium-Binding Proteins

Christen DiPetrillo and Elizabeth Smith

12. Isolation and Analysis of Radial Spoke Proteins

Pooja Kelekar, Mei Wei, and Pinfen Yang

13. Analysis of the Central Pair Microtubule Complex in Chlamydomonas reinhardtii

David R. Mitchell and Brandon Smith