Autophagy in Disease and Clinical Applications, Part C

The third and final installment of Daniel J. Klionsky's new three-volume treatment of autophagy, this volume focuses on monitoring autophagy with regard to disease connections, and presents methods that can be used to analyze autophagy in clinical samples. Edited by one of the leading authorities in the field, this volume and its companion volumes, Autophagy: Lower Eukaryotes and Autophagy in Mammalian Systems, provide a comprehensive overview of the techniques involved in studying autophagy in eukaryotes and simple animal systems, mammalian cells and non-human animals, and humans.

Particularly in times of stress, like starvation and disease, higher organisms have an internal mechanism in their cells for chewing up and recycling parts of themselves. The process of internal “house cleaning” in the cell is called autophagy – literally self-eating. In the future, research in this field will help to design clinical approaches that can turn on autophagy and halt tumor growth.

* Provides an overview of autophagy in regards to humans, specifically regarding disease connections and clinical samples.
* Includes methods to evaluate the role of autophagy in the drug-induced cell death of cancer cells in culture
*Presents reliable methods that, in this relatively new field, allow the reader to find appropriate techniques to identify, monitor, and quantify autophagic processes

1. Initiation of Autophagy by Photodynamic Therapy
Nancy L. Oleinick and David Kessel

2. Autophagic cell death
Michael Lenardo and Li Yu

3. Autophagic neuron death
Yasuo Uchiyama, Masato Koike, Masahiro Shibata, and Mizuho Sasaki

4. Assessing Metabolic Stress and Autophagy Status in Epithelial Tumors
Robin Mathew, Vassiliki Karantza-Wadsworth and Eileen White

5. Autophagic clearance of aggregate–prone proteins associated with neurodegeneration
Sovan Sarkar, Brinda Ravikumar and David Rubinsztein


6. Monitoring Autophagy in Alzheimer's Disease and Related Neurodegenerative Diseases Sovan Dun-Sheng Yang, Ju-Hyun Lee; Ralph A. Nixon


7. Live-cell imaging of autophagy induction and autophagosome-lysosome fusion in primary cultured neurons
Mona Bains and Kim A. Heidenreich

8. Using Genetic Mouse Models to Study the Biology and Pathology of Autophagy in the Central Nervous System
Zhenyu Yue*, Gay R. Holstein, Brian T. Chait and Qing Jun Wang

9. Biochemical and Morphological Detection of Inclusion Bodies in Autophagy-deficient Mice
Masaaki Komatsu and Satoshi Waguri

10. Analyzing Autophagy in Clinical Tissues of Lung and Vascular Diseases
Augustine Choi, Zhi Hua Chen, Hong Pyo Kim and Stefan Ryter

11. Autophagy in Neurite Injury and Neurodegeneration: in vitro and in vivo models
Charleen T. Chu, Edward D. Plowey, Robert W. Hickey, Ruben K. Dagda and Robert S.B. Clark

12. MONITORING THE AUTOPHAGY PATHWAY IN CANCER
John L. Cleveland, Frank Dorsey and Meredith Steeves

13. Autophagy pathways in glioblastoma
Hong Jiang, Erin White, Charles Conrad, Candelaria Gomez-Manzano and Juan Fueyo

14. Autophagy in Lung Cancer
Bo Lu

15. Signal-dependent control of autophagy-related gene expression
Fulvio Chiacchiera and Cristiano Simone

16. Novel Methods for Measuring Cardiac Autophagy in vivo
Roberta A. Gottlieb and Junichi Sadoshima

17. Autophagy in Load-Induced Heart Disease
Hongxin Zhu, Beverly A. Rothermel and Joseph A. Hill

18. EVALUATION OF CELL DEATH MARKERS IN SEVERE CALCIFIED AORTIC VALVES
Wilhelm P. Mistiaen and Michiel W.M. Knaapen

19. Monitoring autophagy in muscle diseases
Ichizo Nishino, May Malicdan and Satoru Noguchi

20. Analyzing Macroautophagy in Hepatocytes and the Liver
Xiao-Ming Yin

21. Monitoring Autophagy in Lysosomal Storage Disorders
Nina Raben, Victoria Hill, Lauren Shea and Paul Plotz