Recent Advances in Cytometry, Part A: Instrumentation, Methods

Cytometry is one of the most rapidly growing methodologies available for basic cell and molecular biology, cytogenetics, immunology, oncology, environmental sciences and also various fields of clinical medicine. This new edition, split into 2 Parts, is an almost completely new book, with nearly all of the chapters devoted to new topics. Like the previous volumes on cytometry published as part of the Methods in Cell Biology series, it provides a comprehensive description of particular cytometric methods and reviews their applications. Chapters present the theoretical foundations of the described methods, their applicability in experimental laboratory and clinical settings, and describes common traps and pitfalls such as problems with data interpretation, comparison with alternative assays, and choosing the optimal assay.

Comprehensive presentation of cytometric methods covering theoretical applications, applicability, potential pitfalls, and comparisions to alternative assaysDiscusses many new assays developed since the previous editionPresents recent developments in cytometric intrumentation/technology

1. Recent advances in Cytometry Instrumentation, Probes and Methods

Anja Mittag, Arkadiusz Pierzchalski, and Attila Tárnok

Section I. Down-sizing cytometry to "micro" dimension

2. Droplet Microfluidics for High-throughput Analysis of Cells and Particles

Michele Zagnoni and Jonathan M. Cooper

3. Parallel Imaging Microfluidic Cytometer

Daniel J. Ehrlich, Brian K. McKenna, James G. Evans, Anna C. Belkina, Gerald V. Denis, David H. Sherr and Man Ching Cheung

4. Microfluidic Systems for Live Cell Imaging

Philip Lee, Terry Gaige and Paul Hung

5. Rise of the micro-machines: microfluidics and the future of cytometry

Donald Wlodkowic and Zbigniew Darzynkiewicz

6. Label-Free Resistive-Pulse Cytometry

M. R. Chapman and L. L. Sohn

Section II. Imaging cytometry

7. Laser Scanning Cytometry and Its Applications: A Pioneering Technology in the Field of Quantitative Imaging Cytometry

Melvin Henriksen, Bruce Miller, Judith Newmark, Yousef Al-Kofahi and Elena Holden

8. Analytical capabilities of ImageStream cytometer

Ewa K. Zuba-Surma and Mariusz Z. Ratajczak

9. Laser scanning cytometry: capturing the immune system in situ

Mairi A McGrath, Angela M Morton and Margaret M Harnett

10. Image cytometry analysis of circulating tumor cells

Lori E. Lowes, David Goodale, Michael Keeney and Alison L. Allan

11. Pre-clinical Applications of Quantitative Imaging Cytometry to Support Drug Discovery

David L Krull and Richard A Peterson

12. Leveraging Image Cytometry for the Development of Clinically Feasible Biomarkers: Evaluation of Activated Caspase-3 in Fine Needle Aspirate Biopsies

Gloria Juan, Stephen J. Zoog and John Ferbas

13. Automation of the buccal micronucleus cytome assay using laser scanning cytometry

Wayne R. Leifert, Maxime François, Philip Thomas, Ed Luther, Elena Holden and Michael Fenech

14. Laser Scanning Cytometry of Mitosis:state and stage analysis

Tammy Stefan and James W. Jacobberger

Section III. Instrumentation, new probes and methods

15. Lasers in Flow Cytometry

William G. Telford

16. The use of hollow fiber membranes combined with cytometry in analysis of bacteriological samples

Kawiak J, Stachowiak R, Lyżniak M, Bielecki J, Granicka L

17. Guide to red fluorescent proteins and biosensors for flow cytometry

Kiryl D. Piatkevich and Vladislav V. Verkhusha

18. Quantum Dot Technology in Flow Cytometry

Pratip K. Chattopadhyay

19. Background-free Cytometry Using Rare Earth Complex Bioprobes

Dayong Jin

20. Surface Enhanced Raman Scattering (SERS) Cytometry

John P. Nolan and David S. Sebba

21. Recent Advances in Flow Cytometric Cell Sorting

Geoffrey W. Osborne