Immunology of Infection

Immunology of Infection, 3e, edited by two leading experts in the field, presents the most appropriate up-to-date experimental approaches in the detail required for modern microbiological research. Focusing on the methods most useful for the microbiologist interested in analyzing host-pathogen relationships, this volume will be essential reading for all researchers working in microbiology, immunology, virology, mycology and parasitology. This new edition of Immunology of Infection provides ready-to-use "recipes" and the latest emerging techniques as well as novel approaches to the tried and tested, established methods included in the successful first edition. Methods in Microbiology is the most prestigious series devoted to techniques and methodology in the field. Established for over 30 years, Methods in Microbiology will continue to provide you with tried and tested, cutting-edge protocols to directly benefit your research.

I. General Methods Radbruch A - Phenotyping and isolation of functionally distinct leukocyte populations; Schütze S - Magnetic isolation of subcellular compartments; Berzofsky J - Prediction of MHC ligands and T-cell epitopes; McMichael A - Quantitative analysis of T-cell responses; Schurr E - Genetics of susceptibility and resistance to infection; Wiker H - Proteomic approaches to study immunity in infection; Sioud M - siRNA as a tool to study immune responses in infection; Schröder JM - Isolation and characterization of antimicrobial peptides; Karin M - Toll - and NOD-like receptors in anti-infective immunity; von Andrian UH - Visualization of immune responses in vivo

II. Murine Models A. In vitro analysis Czuprynski CJ - Isolation of lymphocytes from infected animals; Busch DH - Killer cell assays; Kiyono H - Quantification of murine T-cell cytokine responses; Gordon S - Isolation and functional charactzerization of macrophages

B. In vivo analysis Ehlers S - Measuring immune responses in vivo; Belkaid Y - Control of infection by regulatory T-cells; Stockinger B - Th17 cells in inflammation

C. Specific Models Orme IM - Murine models of tuberculosis; Muller I - The leishmaniasis model; Autenrieth IB - Murine models of infection with extracellular bacteria; Flynn JL - Local immune responses in the lung; Romani L - Murine models of fungal infections; Powrie F - Immunity in the gut; Reddehase MJ - Murine cytomegalovirus infection; Lingnau K/von Gabain A - Boosting of immune responses by adjuvants

III. Human System Banchereau J - Subpopulations of human dendritic cells; Yssel H - Measuring human cytokine responses; Seitzer U - Measuring immune responses in situ; Garcia JV - Humanized mice for the analysis of human immune responses

IV. Appendix Benoist C - The NIH knock-out mouse project; Beutler B - Forward genetic analysis to understand innate immunity