Calcium in Living Cells

Every cell of the body is dependent on calcium to function. Calcium is found in teeth and bones, and calcium signalling is necessary for the movement of muscles and for the action of the heart and the intestines as well as blood coagulation. Calcium in Living Cells will update classic techniques in detecting microscopic levels of calcium ions (Ca2+) in living cells, as well as address new techniques in the field of calcium detection and calcium signaling. Such detection and measurement of intracellular calcium is important to researchers studying the heart, musculoskeletal, gastrointestinal, and immune systems, whose findings will aid in the advancement of drug and genomic therapies to treat heart, gastrointestinal, autoimmune, and infectious diseases.

Gives researchers much needed information on how to study calcium in live cells, which is becoming increasingly important in heart, musculoskeletal, and immune system researchProvides an overview of the latest methods--fluorescence resonance energy transfer (FRET), for example-- that are new to the fieldAllows understanding of how calcium plays a role in intracellular function at the cellular level, which has proved important in Alzheimer's research, heart disease, and areas of musculoskeletal researchUpdated chapters reflect advancements in the classic techniques used'preparing calcium buffers, vibrating the Ca2+ Electrode and confocal imaging

1. A Practical Guide to the Preparation of Ca²⁺ Buffers
   Donald M. Bers, Chris W. Patton and Richard Nuccitelli
2. Photorelease Techniques for Raising or Lowering Intracellular Ca2+
   Robert Zucker
3. Making and Using Calcium-Selective Mini- and Microelectrodes
   L. Hove-Madsen, S. Baudet and D.M. Bers
4. Construction, Theory and Practical Considerations for using Self-Referencing of Ca²⁺-Selective Microelectrodes for Monitoring Extracellular Ca²⁺ Gradients
   Mark A. Messerli and Peter J.S. Smith
5. Practical Aspects of Measuring Intracellular Calcium Signals with Fluorescent Indicators
   Joseph P. Y. Kao, Gong Li, Darryl A. Auston
6. Genetically-encoded probes for measurement of intracellular calcium
   Michael Whitaker
7. Patch clamp methods for studying calcium channels
   David L. Armstrong, Christian Erxleben and Jody A. White
8. Nuclear Patch-Clamp Recording from Inositol 1,4,5-Trisphosphate Receptors
   Taufiq Rahman, Colin W. Taylor
9. Confocal And Multiphoton Imaging Of Intracellular Ca²⁺
   Godfrey Smith, Martyn Reynolds, Francis Burto1, Ole Johan Kemi
10. The Use of Aequorins to Record and Visualize Ca²⁺ Dynamics: From Subcellular Microdomains to Whole Organisms.
    Sarah E. Webb, Kelly L. Rogers, Eric Karplus, and Andrew L. Miller