Translation Initiation: Extract Systems and Molecular Genetics

For over fifty years the Methods in Enzymology series has been the critically aclaimed laboratory standard and one of the most respected publications in the field of biochemistry. The highly relevant material makes it an essential publication for researchers in all fields of life and related sciences. This volume, the first of three on the topic of Translation Initiation includes articles written by leaders in the field.

Chapter 1
Use of Reticulocyte Lysates for Mechanistic Studies of Eukaryotic Translation Initiation
William C. Merrick and Diane Barth-Baus

Chapter 2
Studying translational control in Drosophila cell-free systems
Fátima Gebauer and Matthias W. Hentze

Chapter 3
Use of In Vitro Translation Extract Depleted in Specific Initiation Factors for the Investigation of Translational Regulation
Daniel R. Gallie

Chapter 4
Viral translation and replication in vitro
Yuri V. Svitkin and Nahum Sonenberg

Chapter 5
A practical approach to isolate 48S complexes: Affinity purification and analyses
Nicolas Locker & Peter J. Lukavsky

Chapter 6
Yeast phenotypic assays on translational control
Bumjun Lee, Tsuyoshi Udagawa, Chingakham Ranjit Singh, and Katsura Asano

Chapter 7
Localization and characterization of protein-protein interaction sites
Chingakham Ranjit Singh and Katsura Asano*

Chapter 8
In vivo stabilization of pre-initiation complexes by formaldehyde cross-linking.
Leos Valášek, Bela Szamecz, Alan G. Hinnebusch and Klaus H. Nielsen

Chapter 9
Molecular Genetic Structure-Function Analysis of Translation Initiation Factor eIF5B
Byung-Sik Shin and Thomas E Dever

Chapter 10
The use of fungal in vitro systems for studying translational regulation
Cheng Wu, Nadia Amrani, Allan Jacobson, and Matthew S. Sachs

Chapter 11
Investigating translation initiation using Drosophila molecular genetics
Gritta Tettweiler and Paul Lasko

Chapter 12
Analysis of RNA:protein interactions in vivo: Identification of RNA binding partners of nuclear factor 90
Andrew M. Parrott, Melissa R. Walsh and Michael B. Mathews

Chapter 13
Approaches for Analyzing the Differential Activities and Functions of eIF4E Family Members
Robert E. Rhoads, Tzvetanka D. Dinkova, and Rosemary Jagus

Chapter 14
Tethered function assays: An adaptable approach to study RNA regulatory proteins
Jeff Coller. Mary Wickens

Chapter 15
Analysis of ribosomal shunting during translation initiation in eukaryotic mRNAs
Vincent P. Mauro, Stephen A. Chappell, and John Dresios