Translation Initiation: Reconstituted Systems and Biophysical Methods

For over fifty years the Methods in Enzymology series has been the critically aclaimed laboratory standard and one of the most respected publications in the field of biochemistry. The highly relevant material makes it an essential publication for researchers in all fields of life and related sciences. This volume, the second of three on the topic of Translation Initiation includes articles written by leaders in the field.

Chapter 1
Transient Kinetics, Fluorescence, and FRET in Studies of Initiation of Translation in Bacteria
Pohl Milon, Andrey L. Konevega, Frank Peske, Attilio Fabbretti, Claudio O. Gualerzi, and Marina V. Rodnina

Chapter 2
Binding of mRNA to the Bacterial Translation Initiation Complex
Sean M. Studer and Simpson Joseph

Chapter 3
Real time dynamics of ribosome-ligand interaction by time-resolved chemical probing methods
Attilio Fabbretti, Pohl Milon, Anna Maria Giuliodori, Claudio O. Gualerzi, Cynthia L. Pon

Chapter 4
Over-Expression and Purification of Mammalian Mitochondrial Translational Initiation Factor 2 and Initiation Factor 3
Domenick G. Grasso, Brooke E. Christian, Angela Spencer and Linda L. Spremulli

Chapter 5
In Vitro Studies of Archaeal Translational Initiation
Dario Benelli and Paola Londei

Chapter 6
Reconstitution of yeast translation initiation
Michael G. Acker, Sarah E. Kolitz, Sarah F. Mitchell, Jagpreet S. Nanda and Jon R. Lorsch

Chapter 7
Assembly and analysis of eukaryotic translation initiation complexes
Andrey V. Pisarev, Anett Unbehaun, Christopher U.T. Hellen, and Tatyana V. Pestova

Chapter 8
Reconstitution of Mammalian 48S Ribosomal Translation Initiation Complexes
Romit Majumdar, Jayanta Chaudhuri and Umadas Maitra
Chapter 9
Biophysical approach to studies of cap-eIF4E interaction by synthetic cap analogues
Anna Niedzwiecka, Janusz Stepinski, Jan M. Antosiewicz, Edward Darzynkiewicz and Ryszard Stolarski

Chapter 10
Biophysical studies of the translation initiation pathway using immobilised mRNA analogues
John E.G. McCarthy, Steven Marsden and Tobias von der Haar

Chapter 11
Protection-Based Assays to Measure Amino Acyl-tRNA
Binding to Translation Initiation Factors
Yves Mechulam*, Laurent Guillon, Laure Yatime, Sylvain Blanquet and Emmanuelle Schmitt

Chapter 12
NMR methods for studying protein-protein interactions involved in translation initiation
Assen Marintchev, Dominique Frueh, and Gerhard Wagnerƒx

Chapter 13
Structural methods to study IRES Function
Jeffrey S. Kieft*, David A. Costantino, Megan E. Filbin, John Hammond, and Jennifer S. Pfingsten

Chapter 14
Biophysical and biochemical investigations of dsRNA-activated kinase PKR.
Sean A. McKenna, Darrin A. Lindhout, Takashi Shimoike, and Joseph D. Puglisi

Chapter 15
Expression and purification of recombinant wheat translation initiation factors eIF1, eIF1A, eIF4A, eIF4B, eIF4F, eIF(iso)4F and eIF5
Laura K. Mayberry, Michael D. Dennis, M. Leah Allen, Kelley Ruud Nitka, Patricia A. Murphy, Lara Campbell and Karen S. Browning

Chapter 16
In vitro reconstitution and biochemical characterization of translation initiation by internal ribosomal entry
Victoria G. Kolupaeva, Sylvain de Breyne, Tatyana V. Pestova, Christopher U.T. Hellen