Lipidomics and Bioactive Lipids: Lipids and Cell Signaling

This volume in the well-established Methods in Enzymology series features methods for the study of lipids using mass spectrometry techniques. Articles in this volume cover topics such as Phospholipase A1 assays using a radio-labeled substrate and mass spectrometry; Real-time Cell Assays of Phospholipases A2 Using Fluorogenic Phospholipids; Analysis and Pharmacological Targeting of Phospholipase C â interactions with G proteins; Biochemical Analysis of Phospholipase D.; Measurement of Autotaxin/Lysophospholipase D Activity; Platelet-Activating Factor; Quantitative measurement of PtdIns(3,4,5)P3; Measuring Phosphorylated Akt And Other Phosphoinositide 3-Kinase-Regulated Phosphoproteins In Primary Lymphocytes; Regulation of Phosphatidylinositol 4-Phosphate 5-Kinase activity by partner proteins; Biochemical Analysis of Inositol Phosphate Kinases; Analysis of the phosphoinositides and their aqueous metabolites; Combination of C17-sphingoid base homologues and mass spectrometry analysis as a new approach to study sphingolipid metabolism; Measurement of mammalian sphingosine-1-phosphate phosphohydrolase activity in vitro and in vivo; A rapid and sensitive method to measure secretion of sphingosine-1-phosphate; Ceramide Kinase and Ceramide-1-Phosphate; Measurement of Mammalian Diacylglycerol Kinase Activity in vitro and in Cells; Lipid Phosphate Phosphatases from Saccharomyces cerevisiae.

Chapter 1
Phospholipase A1 assays using a radio-labeled substrate and mass spectrometry
REI MORIKAWA, MASAFUMI TSUJIMOTO, HIROYUKI ARAI and JUNKEN AOKI

Chapter 2
Real-time Cell Assays of Phospholipases A2 Using Fluorogenic Phospholipids
Debasis Manna, Wonhwa Cho

Chapter 3
Analysis and Pharmacological Targeting of Phospholipase C â interactions with G proteins
David M. Lehmann, Chujun Yuan and Alan V. Smrcka

Chapter 4
Biochemical Analysis of Phospholipase D.
H. Alex Brown, Lee G. Henage, Anita M. Preininger, Yun Xiang, John H. Exton

Chapter 5
Measurement of Autotaxin/Lysophospholipase D Activity
Andrew J. Morris and Susan S. Smyth

Chapter 6
Platelet-Activating Factor
John S. Owen, Michael J. Thomas, and Robert L. Wykle

Chapter 7
Quantitative measurement of PtdIns(3,4,5)P3
H. Guillou, L. Stephens, P.T. Hawkins

Chapter 8
Measuring Phosphorylated Akt And Other Phosphoinositide 3-Kinase-Regulated Phosphoproteins In Primary Lymphocytes
Amber C. Donahue, Michael G. Kharas, and David A. Fruman

Chapter 9
Regulation of Phosphatidylinositol 4-Phosphate 5-Kinase activity by partner proteins
Yasunori Kanaho, Kazuhisa Nakayama, Michael A. Frohman, and Takeaki Yokozeki

Chapter 10
Biochemical Analysis of Inositol Phosphate Kinases
James C. Otto, Sashidhar Mulugu, Peter C. Fridy, Shean-Tai Chiou, Blaine N. Armbruster, Anthony A. Ribeiro and John D. York

Chapter 11
Analysis of the phosphoinositides and their aqueous metabolites
Christopher P. Berrie, Cristiano Iurisci, Enza Piccolo, Renzo Bagnati and Daniela Corda

Chapter 12
Combination of C17-sphingoid base homologues and mass spectrometry analysis as a new approach to study sphingolipid metabolism.
Stefka Spassieva, Jacek Bielawski, Viviana Anelli, and Lina M. Obeid

Chapter 13
Measurement of mammalian sphingosine-1-phosphate phosphohydrolase activity in vitro and in vivo
Michael Maceyka, Sheldon Milstien, Sarah Spiegel

Chapter 14
A rapid and sensitive method to measure secretion of sphingosine-1-phosphate
Poulami Mitra, Shawn G. Payne, Sheldon Milstien, Sarah Spiegel

Chapter 15
Ceramide Kinase and Ceramide-1-Phosphate
Dayanjan S. Wijesinghe, Nadia F. Lamour, Antonio Gomez-Munoz, and Charles E. Chalfant

Chapter 16
Measurement of Mammalian Diacylglycerol Kinase Activity in vitro and in Cells
Richard M. Epand and Matthew K. Topham

Chapter 17
Lipid Phosphate Phosphatases from Saccharomyces cerevisiae
George M. Carman and Wen-I Wu