Ribonucleases, Part A: Functional Roles and Mechanisms of Action

This first of two volumes provides up-to-date, methods-related information on ribonuclease functions, assays, and applications. Chapter topics include the identification of, characterization of, and assays for secreted ribonucleases; viral ribonucleases, artificial and engineered ribonucleases, and ribozymes.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Section I: Ribonuclease Classification and Review

[1]: A Natural Classification of Ribonucleases

[2]: The Ribonuclease T1 Family

[3]: Ribonuclease T2

[4]: The Ribonuclease P Family

Section II: Ribunucleases Assays

[5]: Fast, Facile, Hypersensitive Assays for Ribonucleolytic Activity

[6]: Activity Staining for Detection of Ribonucleases Using Dried Agarose Film Overlay Method After Isoelectric Focusing

[7]: Gel Renaturation Assay for Ribonucleases

[8]: Analysis of Ribonucleases following Gel Electrophoresis

[9]: Ribonuclease Assays Utilizing Toluidine Blue Indicator Plates, Methylene Blue, or Fluorescence Correlation Spectroscopy

[10]: Ribonuclease Activities of Trypanosome RNA Editing Complex Directed to Cleave Specifically at a Chosen Site

[11]: Ribonuclease YI*, RNA Structure Studies, and Variable Single-Strand Specificities of RNases

Section III: Secreted Ribonucleases

[12]: Bovine Pancreatic Ribonuclease A: Oxidative and Conformational Folding Studies

[13]: Purification of Engineered Human Pancreatic Ribonuclease

[14]: Degradation of Double-Stranded RNA by Mammalian Pancreatic-Type Ribonucleases

[15]: Seminal Ribonuclease: Preparation of Natural and Recombinant Enzyme, Quaternary Isoforms, Isoenzymes, Monomeric Forms; Assay for Selective Cytotoxicity of the Enzyme

[16]: Angiogenin

[17]: Eosinophil-Derived Neurotoxin

[18]: Eosinophil Cationic Protein

[19]: Deciphering the Mechanism of RNase T1

[20]: Mitogillin and Related Fungal Ribotoxins

[21]: RNase U2 and α-Sarcin: A Study of Relationships

[22]: Secretory Acid Ribonucleases from Tomato, Lycopersicon esculentum Mill.

[23]: Leczyme

Section IV: Ribonucleases H

[24]: Prokaryotic Type 2 RNases H

[25]: RNase H1 of Saccharomyces cerevisiae: Methods and Nomenclature

[26]: Ribonucleases H of the Budding Yeast, Saccharomyces cerevisiae

[27]: Human RNases H

[28]: Assays for Retroviral RNase H

Section V: Synthetic Ribonucleases

[29]: Sequence-Selective Artificial Ribonucleases

[30]: RNA Cleavage by 1,4-Diazabicyclo[2.2.2]octane–Imidazole Conjugates

[31]: Preparation and Use of ZFY-6 Zinc Finger Ribonuclease

Section VI: Ribonucleolytic Nucleic Acids

[32]: RNA Cleavage by the 10-23 DNA Enzyme

[33]: Leadzyme

[34]: Hammerhead Ribozyme Structure and Function in Plant RNA Replication

[35]: Kinetic Analysis of Bimolecular Hepatitis delta Ribozyme

[36]: Catalytic and Structural Assays for the Hairpin Ribozyme

[37]: Intracellular Applications of Ribozymes

Section VII: Ribonuclease Inhibitor

[38]: Barnase–Barstar Interaction

[39]: Cytoplasmic Ribonuclease Inhibitor

[40]: Small Molecule Inhibitors of RNase A and Related Enzymes

[41]: Ribonuclease-Resistant RNA Controls and Standards

Section VIII: Nonenzymatic Cleavage of RNA

[42]: Nonenzymatic Cleavage of Oligoribonucleotides

Author Index

Subject Index