High Resolution Separation and Analysis of Biological Macromolecules, Part A: Fundamentals

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 260 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.

Liquid chromatographyElectrophoresisMass spectrometry

Liquid Chromatography: Methods: M.-I. Aguilar and M.T.W. Hearn, High-Resolution Reversed-Phase High-Performance Liquid Chromatography of Peptides and Proteins. S.-L. Wu and B.L. Karger, Hydrophobic Interaction Chromatography of Proteins. G. Choudhary and C. Horvath, Ion-Exchange Chromatography. M. de Frutos, S.K. Paliwal, and F.E. Regnier, Analytical Immunology. Columns and Instrumentation: M.V. Novotny, Microcolumn Liquid Chromatography in Biochemical Analysis. S.K. Paliwal, M. de Frutos, and F.E. Regnier, Rapid Separations of Proteins by Liquid Chromatography. L.R. Snyder, Automated Method Development in High-Performance Liquid Chromatography. M. Szulc, R. Mhatre, J. Mazzeo, and I.S. Krull, Detection in Liquid Chromatography. G.P. Rozing, Diode Array Detection. Electrophoresis: Slab Gel Electrophoresis: High Resolution: P.G. Righetti, C. Gelfi, and M. Chiari, Isoelectric Focusing in Immobilized pH Gradients. J.C. Wrestler, B.D. Lipes, B.W. Birren, and E. Lai, Pulsed-Field Gel Electrophoresis. G.W. Slater, P. Mayer, and G. Drouin, Migration of DNA through Gels. Capillary Electrophoresis: S. Hjerten, Capillary Electrophoretic Separation in Open and Coated Tubes. N. Matsubara and S. Terabe, Micellar Electrokinetic Chromatography. S. Micinski, M. Gronvald, and B.J. Compton, Structure–Mobility Relationships in Free Solution Zone Electrophoresis. T. Wehr, M. Zhu, and R. Rodriguez-Diaz, Capillary Isoelectric Focusing. L. Krivankova[breve over r], P. Gebauer, and P. Bocek[breve over c], Isotachophoresis. A.W. Moore, Jr., J.P. Larmann, Jr., A.V. Lemmo, and J.W. Jorgenson, Two-Dimensional Liquid Chromatography–Capillary Electrophoresis Techniques for Analysis of Proteins and Peptides. T.T. Lee and E.S. Yeung, Capillary Electrophoresis Detectors: Lasers. Mass Spectrometry: W.E. Seifert, Jr., and R.M. Caprioli, Fast Atom Bombardment Mass Spectrometry. F. Banks, Jr., and C.M. Whitehouse, Electrospray Ionization Mass Spectrometry. R.C. Beavis and B.T. Chait, Matrix-Assisted Laser Desorption Mass–Spectrometry of Proteins. J.C. Schwartz andI. Jardine, Quadrupole Ion Trap Mass Spectrometry. Author Index. Subject Index.