Concepts and Models for Drug Permeability Studies: Cell and Tissue based In Vitro Culture Models

This book intends to be an updated compilation of the most important buccal, gastric, intestinal, pulmonary, nasal, vaginal, ocular, skin and blood-brain barrier in vitro models for predicting the permeability of drugs. Concepts and Models for Drug Permeability Studies focuses on different approaches and comprises of various models. Each model describes the protocol of seeding and conservation, the application for specific drugs, and takes into account the maintenance of physiologic characteristics and functionality of epithelium, from the simplest immortalized cell-based monoculture to the most complex engineered-tissue models. Chapters also discuss the equivalence between in vitro cell and tissue models and in vivo conditions, highlighting how each model may provisionally resemble a different drug absorption route.

Updated information regarding the most recent in vitro models to study the permeability of drugsShort and concise chapters covering all the biological barriers with interest in drug permeabilityA combination of bibliographic information related with individual models and footnote instructions of technical procedures for construction of cell and tissue-based modelsSimple and clear scientific content, adaptable for young scientists and experimented researchers

1. Introduction

2. Importance and applications of cell and tissue-based in vitro models for drug permeability screening in early stages of drug development

3. Immortalized cell-based in vitro culture models for drug permeability studies

4. Primary isolated cell-based in vitro culture models for drug permeability studies

5. Tissue-based in vitro culture models for drug permeability studies

6. Correlation between cell and tissue-based in vitro models for drug permeability screening with in vivo situation: Modeling and functional extrapolation