Mitochondrial Function, Part A: Mitochondrial Electron Transport Complexes and Reactive Oxygen Species

The first of two new volumes covering mitochondria, Mitochondrial Function, Part A presents modern methods that have been developed to examine mitochondrial electron transport chain complexes, iron-sulfur proteins and reactive oxygen species. These new techniques provide investigators with sensitive, original approaches to the study of disease states associated with mitochondrial malfunction.

The critically acclaimed laboratory standard for 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. With more than 400 volumes published, each Methods in Enzymology volume presents material that is relevant in today's labs -- truly an essential publication for researchers in all fields of life sciences.

New methods focusing on the examination of normal and abnormal mitochondrial function are presented in an easy-to-follow format by the researchers who developed themAlong with companion volume covering mitochondrial diseases and defects, provides a comprehensive overview of modern techniques in the study of mitochondrial malfunctionProvides a "one-stop shop" for tried and tested essential techniques, eliminating the need to wade through untested or unreliable methods

Part A: Electron Transport Complexes
1. Visualizing functional flexibility by three dimensional electron microscopy: reconstructing Complex I of the mitochondrial respiratory chain; M. Radermacher
2. Electron tomography of the mitochondrion; T. Frey, G. Perkins, M. Sun
3. Mid-IR vibrational studies of complex I; D. Marshall, P. Rich
4. Electron transfer in respiratory complexes resolved by an ultra-fast freeze-quench approach; N. Belevich, M. Verkhovskya, M. Verkhovsky
5. Use of ruthenium photooxidation techniques to study electron transfer in the cytochrome bc1 complex; F. Millett, B. Durham, C. Yu, L. Yu
6. Use of ruthenium photoreduction techniques to study electron transfer in cytochrome oxidase; F. Millett, B. Durham, R. Gennis
7. Mass spectrometric characterization of subunits of electron transfer complexes that are encoded in the mitochondrial genome; J. Carroll, J. Walker
8. The assembly of respiratory chain complexes studied by GFP-tagged subunits, L. Nijtmans
9. Two-dimensional native electrophoresis for functional assays of mitochondrial complexes; W. Wittig, H. Schägger
10. Reliable assay for complex I in human blood lymphocytes and skin fibroblasts; L. Elly, A. de Wit, W. Sluiter
11. Purification of the cytochrome c reductase/cytochrome c oxidase supercomplex of yeast mitochondria; E. Boekema and H. Braun
12. Supercomplex organization of the yeast mitochondrial respiratory chain complexes and the ADP/ATP carrier proteins; R. Stuart
13. Role of cysteine desulfurase Nfs1 in human mitochondrial Fe-S protein biogenesis; O. Stehling, R. Lill
14. Identification and properties of the novel 2Fe-2S center in the outer membrane protein, mitoNEET; S. Wiley, M. Rardin, J. Dixon
15. Nucleotide-dependent iron-sulfur cluster biogenesis in mitochondria; D. Pain
16. Preparation of samples for EPR and Mössbauer spectroscopy of intact Saccharomyces cerrevisiae mitochondria; J. Garber-Morales, R. Miao, G. Holmes-Hampton, P. Lindahl
17. Fluorescence correlation spectroscopy to probe mitochondrial mobility and intramatrix protein diffusion; P. Willems, W. Koopman
18. Characterization of alternative complex I of Plasmodium falciparum mitochondria; N. Fisher, A. J. Warman, S. Ward, G. Biagini
19. Analysis of respiratory chain complex assembly using radiolabeled nuclear- and mitochondrial-encoded subunits; M. McKenzie, M. Lazarou, M. Ryan

Part B: Reactive Oxygen Species
20. Measuring redox changes to mitochondrial protein thiols using Redox DIGE; T. Hurd, K. Lilley, M. Murphy
21.Measuring the effects of Hepatitis C proteins on mitochondrial electron transport and production of reactive oxygen species; S. Weinman
22. Paraquat induced production of reactive oxygen species in brain mitochondria; D. Drechsel, M. Patel
23. The uptake and interactions of the redox cycler paraquat with mitochondria; H. Cocheme; M. Murphy.
24. Superoxide production by rat brain and skeletal muscle mitochondria; W. Kunz
25. Quantitation, localization, and tissue specificities of mitochondrial reactive oxygen species production; C. Mathews
26. The reaction mechanisms of electron transfer and superoxide generation in the cytochrome nc1 complex; D. Xia, S. Yang, Y. Yin, L. Yu; C. Yu
27. Measurement of superoxide formation by mitochondrial complex I of Yarrowia lipolytica; S. Dröse, A. Galkin, U. Brandt
28. An improved method for introducing point mutations into the mitochondrial cytochrme b gene to facilitate studying the role of cytochrome b in the formation of reactive oxygen species; B. Trumpower