FRET and FLIM Techniques

This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips. Both have become staple techniques in many biological and biophysical fields.

Chapter 1. Förster Resonance Energy transfer –FRET what is it, why do it, and how
it’s done. Robert M. Clegg

Chapter 2.Frequency domain FLIM theory, Instrumentation and data analysis. Peter J.
Verveer and Quentin Hanley.

Chapter 3. Time Domain FLIM; Theory, Instrumentation. Hans C. Gerritsen, A.V.
Agronskaia, A.N. Bader, A. Esposito.

Chapter 4. Multidimensional fluorescence imaging. James James McGinty,
Christopher Dunsby, Egidijus Auksorius, Pieter De Beule, Daniel S. Elson, Neil
Galletly, Oliver Hoffman, Gordon Kennedy, Peter M. P. Lanigan, Ian Munro, Björn
Önfelt, Jose Requejo-Isidro, Klaus Suhling, Clifford B. Talbot, M. John Lever,
Andrew J. deMello, Gordon S. Stamp, Mark A. A. Neil and Paul M. W. French

Chapter 5.Visible fluorescent proteins for FRET. Gert-Jan Kremers and Joachim
Goedhart.

Chapter 6. Small Molecule-based FRET probes. Amanda Cobos Correa, Oliver
Wichmann and Carsten Schultz.

Chapter 7. Filter FRET: quantitative imaging of sensitized emission. Kees Jalink and
Jacco van Rheenen.

Chapter 8. Spectral Imaging and its use in the measurement of Förster Resonance
Energy Transfer in living cells. Steven S. Vogel, Paul. S. Blank, Srinagesh V.
Koushik and Christopher Thaler.

Chapter 9. Total Internal Reflection Fluorescence Lifetime Imaging Microscopy.
Theodorus W.J. Gadella Jr.

Chapter 10. FRET and FLIM applications in plants. Riyaz A. Bhat.

Chapter 11. Biomedical FRET-FLIM applications. Phill B. Jones, Brian J. Bacskai,
Bradley T. Hyman.

Chapter 12. Reflections on FRET imaging: formalism, probes and implementation.
Elizabeth A. Jares-Erijman and Thomas M. Jovin.