Optochemical Biology

Optochemical Biology, Volume 624, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters on a variety of topics, including Cell Lineage Tracing in Zebrafish Embryos with an Expanded Genetic Code, Optical Control of Tumor Induction in the Zebrafish, Optogenetic Control by Pulsed Illumination, Optimizing the Photocontrol of bZIP Coiled Coils with Azobenzene Crosslinkers: Role of the Crosslinking Site, Site-Directed RNA Editing in Vivo Can Be Triggered by the Light-Driven Assembly of an Artificial Riboprotein, In Situ Formation of an Azo Bridge on Proteins Controllable by Visible Light, and much more.

1. Optical control of protein activity and gene expression by photoactivation of caged cyclofen Fatima Hamouri, Weiting Zhang, Isabelle Aujard, Thomas Le Saux, Bertrand Ducos, Sophie Vriz, Ludovic Jullien and David Bensimon 2. Reversible optogenetic control of protein function and localization Daniel Z. Wu, Rachel M. Lackner, Chanat Aonbangkhen, Michael A. Lampson and David M. Chenoweth 3. Protocols for the generation of caged guideRNAs for light-triggered RNA-targeting with SNAP-ADARS Alfred Hanswillemenke and Thorsten Stafforst 4. Combinatorial control of gene function with wavelength-selective caged Morpholinos Sankha Pattanayak, Luis Angel Vázquez-Maldonado, Alexander Deiters and James K. Chen 5. Phosphodiester photo-tethers for the (multi-)cyclic conformational caging of oligonucleotides Patricia Müller, Patrick Seyfried, Anton Frühauf and Alexander Heckel 6. Chemical modification of proteins with photocleavable groups Karthik Nadendla, Bhagyesh Sarode and Simon H. Friedman 7. Intramolecular cross-linking of proteins with azobenzene-based cross-linkers Nobuo Yasuike, Huixin Lu, Peng Xia and G. Andrew Woolley 8. Ruthenium-crosslinked hydrogels for rapid, visible-light protein release Teresa L. Rapp and Ivan J. Dmochowski 9. Chromatically independent, two-color uncaging of glutamate and GABA with one- or two-photon excitation Stefan Passlick and Graham C.R. Ellis-Davies 10. DIY optogenetics: Building, programming, and using the light plate apparatus Karl P. Gerhardt, Sebastian M. Castillo-Hair and Jeffrey J. Tabor 11. Pulsatile illumination for photobiology and optogenetics Julia Dietler, Robert Stabel and Andreas Möglich 12. Genetically encoding photoswitchable click amino acids for general optical control of conformation and function of proteins Christian Hoppmann and Lei Wang 13. Light-activation of Cre recombinase in zebrafish embryos through genetic code expansion Wes Brown and Alexander Deiters