Guide to Yeast Genetics: Functional Genomics, Proteomics, and Other Systems Analysis

This fully updated edition of the bestselling three-part Methods in Enzymology series, Guide to Yeast Genetics and Molecular Cell Biology is specifically designed to meet the needs of graduate students, postdoctoral students, and researchers by providing all the up-to-date methods necessary to study genes in yeast. Procedures are included that enable newcomers to set up a yeast laboratory and to master basic manipulations. This volume serves as an essential reference for any beginning or experienced researcher in the field.

Provides up-to-date methods necessary to study genes in yeastIncludes proceedures that enable newcomers to set up a yeast laboratory and to master basic manipulationsServes as an essential reference for any beginning or experienced researcher in the field

PART I - Functional Genomics 1. Analysis of Gene Function using DNA Microarrays 2. An introduction to microarray data analysis and visualization 3. Genome-wide approaches to monitor pre-mRNA splicing 4. ChIP-Seq: Using high-throughput DNA sequencing for genome-wide identification of transcription factor binding sites 5. Genome-wide mapping of nucleosomes in yeast 6. Genome-wide translational profiling by ribosome footprinting

PART II - Systematic Genetic Analysis 7. Synthetic Genetic Array (SGA) Analysis in Saccharomyces cerevisiae and Schizosaccharomyces pombe 8. Making temperature-sensitive mutants 9. Quantitative Genetic Interaction Mapping using the E-MAP Approach 10. Exploring gene function and drug action using chemogenomic dosage assays

PART III - Proteomics 11. Yeast Expression Proteomics by High-Resolution Mass Spectrometry 12. High-quality binary interactome mapping 13. Quantitative analysis of protein phosphorylation on a system-wide scale by mass spectrometry based proteomics 14. A Toolkit of Protein-fragment Complementation Assays for Studying and Dissecting Large-scale and Dynamic Protein-Protein Interactions in Living Cells 15. Yeast Lipid Analysis and Quantification by Mass Spectrometry 16. Mass Spectrometry-Based Metabolomics of Yeast

PART IV - Systems Analysis 17. Imaging single mRNA molecules in yeast 18. Reconstructing gene histories in Ascomycota fungi 19. Experimental evolution in yeast: a practical guide

PART V - Synthetic Biology 20. Enhancing stress resistance and production phenotypes through transcriptome engineering

PART VI - Advances in Cytology/Biochemistry 21. Visualizing yeast chromosomes and nuclear architecture 22. Quantitative localization of chromosomal loci by immunofluorescence 23. Spinning Disk Confocal Microscopy of Yeast 24. Correlative GFP-immunoelectron microscopy in yeast 25. Analyzing P-bodies and stress granules in Saccharomyces cerevisiae 26. Analyzing mRNA expression using single mRNA resolution fluorescent in situ hybridization 27. The Use of In Vitro Assays to Measure Endoplasmic Reticulum Associated Degradation (ERAD) 28. A protein transformation protocol for introducing yeast prion particles into yeast 29. Overexpression and Purification of Integral Membrane Proteins in Yeast 30. Biochemical, cell biological and genetic assays to analyze amyloid and prion aggregation in yeast

PART VII - Other Fungi 31. Genetics and Molecular Biology in Candida albicans 32. Molecular Genetics of Schizosaccharomyces pombe 33. Applying Genetics and Molecular Biology to the Study of the Human Pathogen Cryptococcus neoformans 34. The Fungal Genome Initiative and lessons learned from genome sequencing 35. Ultradian metabolic cycles in yeast